AI Article Synopsis
- The use of antibiotics in livestock production is contributing to increased antibiotic resistance, particularly from a pathogenic bacterium prevalent on these farms.
- Whole genome sequencing of bacterial isolates from large-scale pig and chicken farms in China revealed three new variants of genomic islands and a hybrid transposon structure related to antibiotic resistance.
- The study identified significant genetic diversity among these strains, highlighting the complex mechanisms of resistance gene acquisition and the presence of rarely found resistance genes.
Article Abstract
The widespread use of antibiotics in large-scale livestock production has led to serious antibiotic resistance. is an important pathogenic bacterium on large-scale farms. Chromosomally localized mobilizable genetic elements (genomic islands) and mobile genetic elements (Tn-like transposons) play an important role in the acquisition and transmission of resistance genes by . To study the prevalence and resistance characteristics of antibiotic-resistant genomic islands in of animal origin in China, we performed whole genome sequencing of isolated from large-scale pig and chicken farms. Three new variants of PmGRI1 (HN31, YN8, and YN9), and a hybrid structure (HN2p) formed by the multidrug-resistant Tn-like-HN2p transposon and a genomic island PmGRI1-HN2p, were identified from . All variants underwent homologous recombination mediated by insertion sequence IS. A genomic rearrangement in the chromosome between the Tn-like-HN2p transposon and PmGRI1-HN2p occurred in HN2p. The heterozygous structure contained various antimicrobial resistance genes, including three copies of fluoroquinolone resistance gene and 16S rRNA methylase gene , which are rarely found in . Our results highlight the structural genetic diversity of genomic islands by characterizing the novel variants of PmGRI1 and enrich the research base of multidrug resistance genomic islands.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8532799 | PMC |
| http://dx.doi.org/10.3390/antibiotics10101268 | DOI Listing |
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