Since the cancellation of broad-spectrum soil-active insecticides in alfalfa ( L.) production, clover root curculio ( F.) (CRC) larval root damage has increased. Current CRC management practices are limited in their ability to suppress larval feeding belowground. First, we field screened developmental alfalfa populations for CRC damage. Subsequently, we developed a soil-less arena to observe nodule feeding and development (head capsule width) of larvae in the lab. This method was used to evaluate five alfalfa populations (two CRC-susceptible (control) and three CRC-resistant populations) against larvae. Further, one CRC-resistant population paired with its genetically similar susceptible population were tested against adult leaf consumption and oviposition in the greenhouse. Field screening revealed that the alfalfa populations selected for little or no larval root feeding damage were more resistant to CRC larval feeding than their corresponding unselected cultivars and significantly more resistant than populations selected for susceptibility. The development of a soil-less arena provided a useful method for evaluation of root-larva interactions. Although larval development was similar across susceptible and resistant alfalfa populations, one CRC-resistant population (NY1713) displayed overall increased nodulation and, thus, had a significantly lower proportion of nodules consumed by larvae. Adult feeding and oviposition aboveground were similar across all populations tested. These results provide possible candidates and screening method for the development and evaluation of alfalfa cultivars that may reduce the impacts of larval feeding and that offer an additional option for CRC management.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537788 | PMC |
| http://dx.doi.org/10.3390/insects12100906 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Pre-breeding in alfalfa germplasm develops highly differentiated populations, as revealed by genome-wide microhaplotype markers.
Sci Rep
January 2025
Plant Science Research Unit, USDA-ARS, St. Paul, MN, USA.
Plant genebanks contain large numbers of germplasm accessions that likely harbor useful alleles or genes absent in commercial plant breeding programs. Broadening the genetic base of commercial alfalfa germplasm with these valuable genetic variations can be achieved by screening the extensive genetic diversity in germplasm collections and enabling maximal recombination among selected genotypes. In this study, we assessed the genetic diversity and differentiation of germplasm pools selected in northern U.
View Article and Find Full Text PDFOptimization of high-throughput marker systems for genomic prediction in alfalfa family bulks.
Plant Genome
March 2025
Agronomy Department, University of Florida, Gainesville, Florida, USA.
Alfalfa (Medicago sativa L.) is a perennial forage legume esteemed for its exceptional quality and dry matter yield (DMY); however, alfalfa has historically exhibited low genetic gain for DMY. Advances in genotyping platforms paved the way for a cost-effective application of genomic prediction in alfalfa family bulks.
View Article and Find Full Text PDFHoneysuckle branches and lactobacillus enhance quality by changing the composition and diversity of microorganisms in alfalfa silage fermentation.
Heliyon
November 2024
Ningxia animal husbandry workstation, Yinchuan, 750002, China.
The incorporation of honeysuckle as a silage additive in alfalfa production has yielded promising results; however, the underlying microbial mechanisms during fermentation remain poorly understood. This study leveraged high-throughput sequencing and nutrient profiling to elucidate microbial population dynamics over 45 days of anaerobic fermentation within a vacuum-assisted co-culture system comprising varying fresh weight ratios of honeysuckle branches, , and alfalfa. The experimental design encompassed a -supplemented treatment (M group) and an unsupplemented control (R group), each with five honeysuckle percentages (5 %, 10 %, 15 %, 20 %, 25 %) and respective negative controls.
View Article and Find Full Text PDFHow colonizing alfalfa sprouts modulates the virulence of Shiga toxin-producing Escherichia coli.
Int J Food Microbiol
January 2025
Department of Food Science and Technology, The Ohio State University, Columbus, OH, USA; Department of Microbiology, The Ohio State University, Columbus, OH, USA. Electronic address:
Shiga toxin-producing Escherichia coli (STEC), a significant cause of foodborne illnesses, is often associated with the consumption of fresh produce, including alfalfa sprouts. This study was executed to determine how quickly STEC grows, adapts, and colonizes alfalfa sprouts during production and storage, and whether the pathogen's virulence and infectious doses are affected by physiological adaptation to sprouts as an environment. A reporter STEC O157:H7 EDL933 strain was developed to track the transcription of eae, a virulence gene involved in colonizing human intestinal enterocytes.
View Article and Find Full Text PDFStructure, relationships and diversity in the community of aphids and aphidophagous species in alfalfa.
Bull Entomol Res
November 2024
Department 'Forage production and breeding', Agricultural Academy, Institute of Forage Crops, 5800 Pleven, Bulgaria.
There is a need for comprehensive research on the species structure and the population dynamics of the most common aphidophagous species. A critical factor of the effectiveness of aphid biocontrol is the ratio of beneficial polyphagous (generalist) to oligo- or monophagous (specialist) species within the various trophic groups. Aphids' population density and environmental conditions influence the development and potential feeding of useful insects.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!