Since salivary chromogranin A (CgA) is one of the known sympathetic adrenomedullar system (SAM) stress markers in humans and pigs, this study aimed to investigate whether salivary CgA in dogs reflects SAM activation. Our hypothesis was that salivary CgA would increase when central noradrenaline was pharmacologically induced. A selective noradrenaline transporter blocker, atomoxetine, was orally administered without causing any aversive responses in nine laboratory dogs to see if it would increase salivary CgA. Three treatment groups (i.e., atomoxetine, placebo, and pre-administration of a selective alpha-2 adrenoreceptor agonist (dexmedetomidine) followed by atomoxetine) were prepared with a randomized crossover design. Saliva sample collection, heart rate measurement and behavior observation were performed at Time 0 (baseline) and at 30, 60, 90 and 150 min after each treatment administration. The results demonstrated that salivary CgA significantly increased at 90 min in the atomoxetine treatment ( < 0.05), whereas it was not observed in the other two treatments. The present study showed that salivary CgA was increased by atomoxetine-induced SAM activation. However, this increase was blocked if dexmedetomidine was pre-administered. Overall, the results indicate that salivary CgA is a potential candidate for SAM-mediated stress markers in dogs. Further study to determine the dynamics of salivary CgA will be helpful in its practical use.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8532795 | PMC |
| http://dx.doi.org/10.3390/ani11102844 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Dietary fiber and weaning age affect stress and immune markers in saliva of sows and their offspring.
J Anim Sci
January 2024
Institute of Animal Nutrition, Freie Universität Berlin, Berlin 14195, Germany.
Article Synopsis
- - The study investigated how different diets (hay vs. sugar beet pulp) and grinding sizes (fine vs. coarse) during sow gestation and lactation affect stress and immune markers in both sows and their piglets, as well as the impact of weaning age (conventional vs. late).
- - Salivary markers such as chromogranin A (CgA), alpha-amylase, and oxytocin were notably influenced by diet and weaning age, with specific increases observed in sows and piglets fed certain types of fiber at various weaning stages.
- - Overall, the findings indicate that dietary fiber type, weaning age, and grinding size significantly influence stress and immune responses in pigs,
Quantification of Salivary Chromogranin A Levels during Routine Dental Procedures in Children: An Study.
Int J Clin Pediatr Dent
May 2024
Department of Pediatric and Preventive Dentistry, Bharati Vidyapeeth Dental College and Hospital, Bharati Vidyapeeth (Deemed to be University), Pune, Maharashtra, India.
Background: Routine dental procedures frequently involve invasive treatment, multiple injections, and the use of sharp, high-speed cutting instruments, and treatment is often extended over several visits, which are said to be stress-provoking events. Salivary chromogranin A (CgA) is a biomarker that can help clinicians evaluate and quantify the stress experienced by a child during these procedures.
Aim: To quantify the changes in salivary CgA levels in children before, during, and after routine dental procedures for multiple appointments.
Changes in cortisol, cortisone and 11β-hydroxysteroid dehydrogenase type II activity in saliva during pregnancy and lactation in sows.
Domest Anim Endocrinol
October 2024
Department of Animal and Food Science, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Barcelona, Spain.
The activity of the enzyme 11β-hydroxysteroid dehydrogenase type II (11β-HSD type II), which can be estimated by the combined measurement of cortisol and cortisone, is gaining importance as a marker for the assessment of stress in pigs. The aim of this study was to investigate the activity of this enzyme and the salivary concentrations of cortisol and cortisone in pigs during pregnancy, farrowing and lactation and to compare it with other stress-related biomarkers such as Chromogranin A (CgA), S100A12 and alpha-amylase. Salivary cortisone concentrations and 11β-HSD type II activity decreased after farrowing, while cortisol concentrations increased.
View Article and Find Full Text PDFImpact of chlorogenic acid on submandibular salivary gland and liver of albino rats exposed to sodium nitrite.
BMC Oral Health
August 2024
Department of Oral Pathology, Faculty of Dentistry, Mansoura University, Mansoura, Egypt.
Aim: The aim of the present study is to show how sodium nitrite alters the histology of submandibular salivary glands and livers of Albino rats, as well as how chlorogenic acid may have therapeutic benefits.
Methods: A sample size of thirty male Sprague Dawley Albino rats weighing between 100 and 150 g (5-6 weeks old) was randomly allocated into 3 equal groups. Group I: rats were used as controls and were given phosphate buffer solution, whereas Group II: rats were given an 80 mg/kg sodium nitrites (SN) daily dissolved in distilled water.
Determination of an Acclimation Period for Swine in Biomedical Research.
J Am Assoc Lab Anim Sci
November 2024
Swine are widely used models in biomedical research due to their physiologic and anatomic similarities to humans. During transport from vendors to research facilities, pigs are subject to a number of stressors, including environmental, social, and stress as a result of deprivation from food and water. As stress can have a number of adverse psychologic and physiologic effects, an acclimation period, defined as the period of time that an animal has to adjust and stabilize in a new environment, is recommended.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!