Background: Renal cell carcinoma (RCC) is a common malignant tumor of the urinary system that seriously threatens human life and health. This study aims to explore the role of the traditional Chinese medicine in the pathogenesis of RCC. Meanwhile, this study also revealed the molecular biological mechanism of its antitumor activity.
Methods: Human RCC 786-O cells were cultured in the RPMI-1640 medium, which contains different concentrations of (1,000, 3,000, and 9,000 g/ml). MTT and flow cytometry assays were used to detect the viability of 786-O cells. Transwell and wound healing assays were used to detect cell metastasis. The protein expression was observed by western blot analysis.
Results: can inhibit cell proliferation and induce apoptosis in RCC 786-O cells. In addition, can weaken the migration and invasion of 786-O cells. More importantly, can block the mTOR pathway by inhibiting the protein expression of p-mTOR. Moreover, the high concentration of has a better inhibitory effect on 786-O cells.
Conclusion: The traditional Chinese medicine inhibits the viability and metastasis of 786-O cells by blocking the mTOR pathway.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8526199 | PMC |
| http://dx.doi.org/10.1155/2021/5122380 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Overexpression of PLCG2 and TMEM38A inhibit tumor progression in clear cell renal cell carcinoma.
Sci Rep
January 2025
Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, China.
Clear cell renal cell carcinoma is a prevalent urological malignancy, imposing substantial burdens on both patients and society. In our study, we used bioinformatics methods to select four putative target genes associated with EMT and prognosis and developed a nomogram model which could accurately predicting 5-year patient survival rates. We further analyzed proteome and single-cell data and selected PLCG2 and TMEM38A for the following experiments.
View Article and Find Full Text PDFSensitivity of renal cell carcinoma to cuproptosis: a bioinformatics analysis and experimental verification.
J Cancer
January 2025
Laboratory of Stem Cell Regulation with Chinese Medicine and Its Application, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China.
Targeting cuproptosis is considered as a promising therapeutic strategy for the prevention of tumors. However, the potential role of cuproptosis and its related genes in clear cell renal cell carcinoma (ccRCC) remains elusive. The present study aims to explore the sensitivity of ccRCC to cuproptosis and its underlying mechanism.
View Article and Find Full Text PDFCASP5 associated with PANoptosis promotes tumorigenesis and progression of clear cell renal cell carcinoma.
Cancer Cell Int
January 2025
Institute for Genome Engineered Animal Models of Human Diseases, National Center of Genetically Engineered Animal Models for International Research, Dalian Medical University, 9 West Section Lvshun South Road, Dalian, 116044, China.
Clear cell renal cell carcinoma (ccRCC) is a globally severe cancer with an unfavorable prognosis. PANoptosis, a form of cell death regulated by PANoptosomes, plays a role in numerous cancer types. However, the specific roles of genes associated with PANoptosis in the development and advancement of ccRCC remain unclear.
View Article and Find Full Text PDFElucidating the anticancer potential of dendrobine in renal cell carcinoma treatment.
Naunyn Schmiedebergs Arch Pharmacol
January 2025
Department of Urology, Tongren Hospital Shanghai Jiao Tong University School of Medicine, No.1111 Xian Xia Road, Shanghai, 200336, China.
Renal cell carcinoma (RCC) is the predominant form of kidney cancer. Despite the significant improvements in survival rates for advanced RCC patients due to targeted therapy and immunotherapy, challenges such as drug resistance and severe adverse reactions continue to hinder effective management. Therefore, there is an urgent need to identify new therapeutic agents for RCC.
View Article and Find Full Text PDFDiscovery and optimization of 1,2,4-triazole derivatives as novel ferroptosis inhibitors.
Eur J Med Chem
February 2025
Basic Medicine Research and Innovation Center for Novel Target and Therapeutic Intervention (Ministry of Education), Department of Urology and Department of Cancer Center of the Second Affiliated Hospital, College of Pharmacy, Chongqing Medical University, Chongqing, 400016, China. Electronic address:
Ferroptosis is a novel form of regulated cell death characterized by iron-dependent lipid ROS accumulation, which is associated with various diseases, including acute organ injury, neurodegenerative disorders, and cancer. Pharmacological inhibition of ferroptosis has great potential for the treatment of these diseases. However, the clinical translation of many ferroptosis inhibitors is hindered by their inadequate activity or suboptimal pharmacokinetic profiles.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!