AI Article Synopsis
- The study aimed to create a system for inducing polyploid giant cancer cells (PGCCs) and to explore their characteristics and clinical importance in laryngeal cancer.
- Researchers used paclitaxel liposomes to induce PGCCs and assessed protein expression related to cancer progression and stem cell markers through western blot analysis.
- Results indicated that the induction was successful, with significant changes in protein expression and a negative correlation between PGCC count and patient survival, suggesting PGCCs may contribute to a worse prognosis in laryngeal cancer patients.
Article Abstract
Objectives: We aimed to construct an induction system for polyploid giant cancer cells (PGCCs), as well as to investigate PGCC features and clinical significance.
Methods: A laryngeal neoplasm-PGCC induction system was constructed using paclitaxel liposomes (PTX). We used western blots to compare expression of epithelial-mesenchymal transition-related proteins, stem cell interrelated proteins, and cyclin-associated proteins. We then measured PGCC count in tissue samples of patients with laryngeal neoplasms and analyzed its relationship with prognosis. Statistical significance was determined using -tests.
Results: PTX successfully induced PGCCs. Western blotting showed that CyclinB1, CDC25C, CDK1, E-cadherin, and EIF-4A expression decreased in PGCCs compared with normal cancer cells, whereas vimentin and CD133 expression increased. Number of PGCCs in laryngeal cancer tissues and overall survival time were inversely correlated ( < .05).
Conclusions: PTX successfully induces PGCC formation in laryngeal carcinoma, which may be the cause of poor prognosis in patients with laryngeal cancer.Level of Evidence: 4.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8513447 | PMC |
| http://dx.doi.org/10.1002/lio2.667 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!