L-Tryptophan is known as an aromatic amino acid and one of the essential amino acids that must be ingested through various additives or food. TrpCF is a bifunctional enzyme that has indole-glycerol-phosphate synthase (IGPS) and phosphoribosylanthranilate isomerase (PRAI) activity. In this report, we identified the crystal structure of TrpCF from (TrpCF) and successfully elucidated the active site by attaching rCdRP similar to the substrate and product of the TrpCF reaction. Also, we revealed that TrpCF shows a conformational change at the loops upon substrate binding. We analyzed amino acid sequences of the homologues of TrpCF, and the residues of the substrate-binding site in TrpCF were highly conserved except for some residues. These less conserved residues were replaced by site-directed mutagenesis experiments. Consequently, we obtained the TrpCF (PRAI) variant that has enhanced activity.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acs.jafc.1c05132 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Metal-Assisted Synthesis of Diverse Porphyrinoids by Cyclization of an N-Confused Thia-Pentapyrrane.
Chem Asian J
January 2025
East China University of Science and Technology, Institute of Fine Chemicals, Meilong Road, 200237, Shanghai, CHINA.
Oxidation of thia-pentapyrrane S-P4 with terminal β-linked pyrrole and thiophene units in the presence of various metal ions has been found to afford distinct porphyrinoids. Specifically, N-confused thiasapphyrin (1), Cu(III) norrole (2), neo-confused phlorin (3), and p-benzinorrole (4) were obtained, when S-P4 was oxidized with p-chloranil in acetonitrile in the presence of Ni2+, Cu2+, Cd2+, and Co2+, respectively. The structures of 1-4 have been clearly elucidated by NMR spectroscopy, HRMS, and X-ray crystal diffraction (for 2-4).
View Article and Find Full Text PDFEngineered protein G variants for multifunctional antibody-based assemblies.
Protein Sci
February 2025
Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois, USA.
We have developed a portfolio of antibody-based modules that can be prefabricated as standalone units and snapped together in plug-and-play fashion to create uniquely powerful multifunctional assemblies. The basic building blocks are derived from multiple pairs of native and modified Fab scaffolds and protein G (PG) variants engineered by phage display to introduce high pair-wise specificity. The variety of possible Fab-PG pairings provides a highly orthogonal system that can be exploited to perform challenging cell biology operations in a straightforward manner.
View Article and Find Full Text PDFSensitive dependence of pairing symmetry on Ni-e crystal field splitting in the nickelate superconductor LaNiO.
Nat Commun
January 2025
NYU-ECNU Institute of Physics, NYU Shanghai, Shanghai, China.
The discovery of high-temperature superconductivity in LaNiO under pressure has drawn great attention. However, consensus has not been reached on its pairing symmetry in theory. By combining density-functional-theory (DFT), maximally-localized-Wannier-function, and linearized gap equation with random-phase-approximation, we find that the pairing symmetry of LaNiO is d, if its DFT band structure is accurately reproduced by a downfolded bilayer two-orbital model.
View Article and Find Full Text PDFWater-regulated viscosity-plasticity phase transitions in a peptide self-assembled muscle-like hydrogel.
Nat Commun
January 2025
Department of Chemistry, School of Science, Westlake University, Hangzhou, Zhejiang Province, China.
The self-assembly of small molecules through non-covalent interactions is an emerging and promising strategy for building dynamic, stable, and large-scale structures. One remaining challenge is making the non-covalent interactions occur in the ideal positions to generate strength comparable to that of covalent bonds. This work shows that small molecule YAWF can self-assemble into a liquid-crystal hydrogel (LCH), the mechanical properties of which could be controlled by water.
View Article and Find Full Text PDFSuppression of TLR4/NF-κB signaling by kaurenoic acid in a mice model of monosodium urate crystals-induced acute gout.
Arch Biochem Biophys
January 2025
Pharmacological Sciences Research Lab, Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan; Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan. Electronic address:
Aim: The aim of the current study was to investigate the potential therapeutic effect of kaurenoic acid (KA) against Monosodium Urate Crystals (MSU)- induced acute gout by downregulation of NF-κB signaling pathway, mitigating inflammation and oxidative stress produced by MSU crystals. KA potentially targeted NF-κB pathway activation and provided comprehensive insights through multiple approaches. This was accomplished by advanced analytical techniques.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!