AI Article Synopsis
- Recent increases in cheese consumption and the rising cost of traditional rennet have sparked interest in finding plant-based alternatives for cheese production, particularly for those with dietary restrictions.
- This study investigates an aspartic protease from Salpichroa origanifolia fruits (SoAP), which was found to have lower milk-clotting capabilities compared to animal rennet but showed greater hydrolysis of α-casein.
- The identification of several bioactive peptides from different casein types suggests that SoAP could not only serve as an alternative rennet but also offer health benefits, making it suitable for artisan cheese manufacturing.
Article Abstract
In recent years, many attempts have been made to find new plant proteases to make artisan cheeses. The global increase in cheese consumption, together with a lower supply and increasing cost of calf rennet, religious factors (Islam and Judaism) and food choices (vegetarianism) have led to the search for suitable rennet substitutes for milk clotting. This study describes the milk-clotting and hydrolytic activities of an aspartic protease from Salpichroa origanifolia fruits (SoAP) on individual caseins to explore its potential use as an alternative to animal rennet. The milk-clotting index obtained for SoAP was 8.4 times lower than that obtained for chymosin. SoAP showed a higher degree of hydrolysis on α-casein than on the other fractions under the proposed conditions. RP-HPLC, mass spectrometry analyses and sequencing of the hydrolysates allowed identifying five peptides from α-casein, one peptide from β-casein, and three peptides from k-casein. In silico analysis showed that the peptides identified may display a wide variety of potential biological activities. These results demonstrate the possibility of using SoAP for the manufacture of new types or artisan cheeses, with the simultaneous added value of the potential health-promoting benefits of the bioactive peptides generated during the hydrolysis.
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| http://dx.doi.org/10.1016/j.ijbiomac.2021.10.004 | DOI Listing |
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Article Synopsis
- Recent increases in cheese consumption and the rising cost of traditional rennet have sparked interest in finding plant-based alternatives for cheese production, particularly for those with dietary restrictions.
- This study investigates an aspartic protease from Salpichroa origanifolia fruits (SoAP), which was found to have lower milk-clotting capabilities compared to animal rennet but showed greater hydrolysis of α-casein.
- The identification of several bioactive peptides from different casein types suggests that SoAP could not only serve as an alternative rennet but also offer health benefits, making it suitable for artisan cheese manufacturing.
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