AI Article Synopsis
- Cryptochromes (CRYs) are light-sensitive proteins that help regulate circadian clocks in both plants and animals by affecting mRNA modification.
- The study reveals that CRY2 plays a critical role in modifying over 10% of mRNAs in Arabidopsis, particularly those influenced by the circadian clock, by interacting with key protein components involved in mA methylation.
- Mutations in CRY and mTA genes lead to similar issues like extended circadian cycles and diminished mRNA stability, suggesting that light-induced processes involving CRYs are essential for proper circadian regulation and mRNA management in plants.
Article Abstract
Cryptochromes (CRYs) are photoreceptors that mediate light regulation of the circadian clock in plants and animals. Here we show that CRYs mediate blue-light regulation of N-methyladenosine (mA) modification of more than 10% of messenger RNAs in the Arabidopsis transcriptome, especially those regulated by the circadian clock. CRY2 interacts with three subunits of the METTL3/14-type N-methyladenosine RNA methyltransferase (mA writer): MTA, MTB and FIP37. Photo-excited CRY2 undergoes liquid-liquid phase separation (LLPS) to co-condense mA writer proteins in vivo, without obviously altering the affinity between CRY2 and the writer proteins. mta and cry1cry2 mutants share common defects of a lengthened circadian period, reduced mA RNA methylation and accelerated degradation of mRNA encoding the core component of the molecular oscillator circadian clock associated 1 (CCA1). These results argue for a photoregulatory mechanism by which light-induced phase separation of CRYs modulates mA writer activity, mRNA methylation and abundance, and the circadian rhythms in plants.
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| http://dx.doi.org/10.1038/s41477-021-01002-z | DOI Listing |
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