Ethnopharmacological Relevance: Previous studies have shown that the active fraction of Rhodiola tangutica (Maxim.) S.H. Fu (ACRT) dilates pulmonary arteries and thwarts pulmonary artery remodelling. The dilatation effect of ACRT on pulmonary artery vascular rings could be reduced by potassium (K) channel blockers. However the exact mechanisms of ACRT on ion channels are still unclear.
Aim Of The Study: This study aimed to investigate whether the effect of ACRT on K channels inhibits cell proliferation after pulmonary artery smooth muscle cells (PASMCs) are exposed to hypoxia.
Materials And Methods: The whole-cell patch-clamp method was used to clarify the effect of ACRT on the K current (I) of rat PASMCs exposed to hypoxia. The mRNA and protein expression levels were detected using real-time quantitative polymerase chain reaction (RT-qPCR) and western blotting, respectively. The intracellular calcium (Ca) concentration ([Ca]) values in rat PASMCs were detected by laser scanning confocal microscopy. The cell cycle and cell proliferation were assessed using flow cytometry analysis and CCK-8 and EdU assays.
Results: ACRT pretreatment alleviated the inhibition of I induced by hypoxia in rat PASMCs. Compared with hypoxia, ACRT upregulated voltage-dependent K channel (K) 1.5 and big-conductance calcium-activated K channel (BK) mRNA and protein expression and downregulated voltage-dependent Ca channel (Ca) 1.2 mRNA and protein expression. ACRT decreased [Ca], inhibited the promotion of cyclin D1 and proliferating cell nuclear antigen (PCNA) expression, and prevented the proliferation of rat PASMCs exposed to hypoxia.
Conclusion: In conclusion, the present study demonstrated that ACRT plays a key role in restoring ion channel function and then inhibiting the proliferation of PASMCs under hypoxia, ACRT has preventive and therapeutic potential in hypoxic pulmonary hypertension.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jep.2021.114734 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effect of breathing exercises to prevent pulmonary complications in patients undergoing coronary artery bypass graft surgery: a prospective randomized controlled trials study protocol.
Front Med (Lausanne)
January 2025
Xuanwu Hospital, Capital Medical University, Beijing, China.
Purpose: To study the effects of breathing exercises on preventing pulmonary complications in patients undergoing coronary artery bypass graft surgery.
Methods: Observing whether preoperative breathing exercises can reduce the incidence of postoperative pulmonary complications in patients undergoing coronary artery bypass graft surgery; observing whether these exercises can improve postoperative arterial oxygen pressure, oxygen saturation, and the distance walked in a six-minute walk test after surgery; as well as reduce hospital stay duration, lower treatment costs, and improve the quality of life as measured by the Short Form-36 Health Survey (SF-36).
Design: The study population includes patients undergoing coronary artery bypass graft surgery under general anesthesia; the research center is Capital Medical University Xuanwu Hospital; the sample size is 120.
PDA-associated infective endocarditis with pulmonary artery perforation.
Pak J Med Sci
January 2025
Muhammad Ali Mumtaz, MD FACS. Tahir Heart Institute, Fazl-e-Omar Hospital, Chenab Nagar, District Chiniot, Pakistan.
Infective endocarditis used to frequently cause mortality in subjects having PDA before the advent of antibiotics and surgical ligation. It has been documented that clinically silent PDAs may cause infective complications of heart valves. We present case of an 18-years-old male who presented with palpitations and fever to our emergency department.
View Article and Find Full Text PDFPredicting Extended Intensive Care Unit Stay Following Coronary Artery Bypass Grafting and Its Impact on Hospitalization and Mortality.
J Clin Med Res
January 2025
Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan.
Background: Coronary artery bypass grafting (CABG) is a prevalent surgical procedure aimed at alleviating symptoms and improving survival in patients with coronary artery disease (CAD). Postoperative care typically necessitates an intensive care unit (ICU) stay, which is ideally less than 24 h. However, various preoperative, intraoperative, and postoperative factors can prolong ICU stays, adversely affecting hospital resources, patient outcomes, and overall healthcare costs.
View Article and Find Full Text PDFImpact of sodium-glucose cotransporter-2 inhibitors on pulmonary vascular cell function and arterial remodeling.
World J Cardiol
January 2025
Chinese Academy Medical Sciences, Fuwai Yunnan Hospital, Kunming 650000, Yunnan Province, China.
Sodium-glucose cotransporter-2 (SGLT-2) inhibitors represent a cutting-edge class of oral antidiabetic therapeutics that operate through selective inhibition of glucose reabsorption in proximal renal tubules, consequently augmenting urinary glucose excretion and attenuating blood glucose levels. Extensive clinical investigations have demonstrated their profound cardiovascular efficacy. Parallel basic science research has elucidated the mechanistic pathways through which diverse SGLT-2 inhibitors beneficially modulate pulmonary vascular cells and arterial remodeling.
View Article and Find Full Text PDFIdiopathic pulmonary arteriovenous malformation: a rarity in clinical practice.
J Vasc Bras
January 2025
Universidade Federal da Paraíba - UFPB, Hospital Universitário Lauro Wanderley - HULW, João Pessoa, PB, Brasil.
Pulmonary arteriovenous malformations (PAVM) are characterized by abnormal pulmonary vessels forming arteriovenous shunts that compromise oxygenation of the blood, causing hypoxemia, and predispose to infections and cerebral ischemia. The patient in this case was a 38-year-old male who presented with tachypnea and dyspnea, cyanosis of extremities, and significant digital clubbing. The patient had structural epilepsy secondary to neurosurgery for a cerebral abscess during childhood.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!