Biosci Biotechnol Biochem
Department of Sensory Physiology, Asahikawa Medical University, Asahikawa, Hokkaido, Japan.
Published: November 2021
We developed a rapid and accurate method for quantifying gaseous phase odorants using headspace solid-phase microextraction (HS-SPME) in conjunction with GC-MS and used our system to quantify alkylpyrazine analogs in the Y-maze. Rapid extraction of volatile compounds in the vapor phase achieved accurate quantitative analysis of gaseous alkylpyrazine analogs at several locations in the Y-maze. We also used a series of three SPME fibers to quantify changes in the concentration over time. We conducted a behavioral test of mice in response to these alkylpyrazines and identified a positive relationship between the rate of increase in gaseous concentration and the avoidance rate induced. Our results demonstrate that the Y-maze is a simple but reliable apparatus for behavioral studies of olfaction. The HS-SPME fast extraction method can quantify how gaseous concentrations of alkylpyrazines change over time, and the time-dependent increase of alkylpyrazine concentration is correlated with induction of aversive behavior in mice.
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http://dx.doi.org/10.1093/bbb/zbab178 | DOI Listing |
J Agric Food Chem
August 2024
Department of Food Science, Rutgers University, 65 Dudley Road, New Brunswick, New Jersey 08901, United States.
The formation pathway and mechanism of various pyrazines were investigated during the thermal treatment of the alanine-xylose Amadori compound (Ala-ARP) and exogenous alanine (Ala). -labeled Ala was used to coheated with Ala-ARP to clarify the nitrogen sources and the respective contributions of exogenous Ala and the regenerated Ala released from Ala-ARP to different pyrazine formation. It was found that exogenous Ala exhibited a priority in capturing glyoxal (GO) to form pyrazine during the thermal degradation of ARP.
View Article and Find Full Text PDFBiosci Biotechnol Biochem
November 2021
Department of Sensory Physiology, Asahikawa Medical University, Asahikawa, Hokkaido, Japan.
We developed a rapid and accurate method for quantifying gaseous phase odorants using headspace solid-phase microextraction (HS-SPME) in conjunction with GC-MS and used our system to quantify alkylpyrazine analogs in the Y-maze. Rapid extraction of volatile compounds in the vapor phase achieved accurate quantitative analysis of gaseous alkylpyrazine analogs at several locations in the Y-maze. We also used a series of three SPME fibers to quantify changes in the concentration over time.
View Article and Find Full Text PDFMicrobiome
January 2021
Institute of Environmental Biotechnology, Graz University of Technology, Graz, Austria.
Background: Antimicrobial resistance (AMR) is a major threat to public health. Microorganisms equipped with AMR genes are suggested to have partially emerged from natural habitats; however, this hypothesis remains inconclusive so far. To understand the consequences of the introduction of exogenic antimicrobials into natural environments, we exposed lichen thalli of Peltigera polydactylon, which represent defined, highly diverse miniature ecosystems, to clinical (colistin, tetracycline), and non-clinical (glyphosate, alkylpyrazine) antimicrobials.
View Article and Find Full Text PDFAppl Environ Microbiol
December 2019
Key Laboratory of Industrial Biotechnology of Ministry of Education, State Key Laboratory of Food Science and Technology, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, China
Alkylpyrazines are important contributors to the flavor of traditional fermented foods. Here, we studied the synthesis mechanisms of 2,5-dimethylpyrazine (2,5-DMP) and 2,3,5-trimethylpyrazine (TMP). Substrate addition, whole-cell catalysis, stable isotope tracing experiments, and gene manipulation revealed that l-threonine is the starting point involving l-threonine-3-dehydrogenase (TDH) and three uncatalyzed reactions to form 2,5-DMP.
View Article and Find Full Text PDFMol Nutr Food Res
July 2019
Department of Chemistry, Division of Food Chemistry and Toxicology, Technische Universität Kaiserslautern, Erwin-Schrödinger-Str. 52, D-67663, Kaiserslautern, Germany.
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