Polyethersulfone (PES) membranes provide a versatile tool for gravity-related plant studies. Benefits of this system include straightforward setup, no need for specialized equipment, long-term seed viability between plating and hydration/growth, and adaptability to diverse protocols and downstream analyses. Methods outlined here include seed sterilization, planting, growth, and dissection that will transition directly into any RNA extraction protocol.
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http://dx.doi.org/10.1007/978-1-0716-1677-2_15 | DOI Listing |
Int J Mol Sci
January 2025
Medical Research Core Facility and Platforms (MRCFP)-Drug Discovery Platform, King Abdullah International Medical Research Center (KAIMRC), King Saud Bin Abdulaziz University for Health Sciences, Ministry of National Guard Health Affairs (MNGHA), Riyadh 11481, Saudi Arabia.
Peroxisome proliferator-activated receptors (PPARs) are considered good drug targets for breast cancer because of their involvement in fatty acid metabolism that induces cell proliferation. In this study, we used the KAIMRC1 breast cancer cell line. We showed that the PPARE-Luciferase reporter gets highly activated without adding any exogenous ligand when PPAR alpha is co-transfected, and the antagonist GW6471 can inhibit the activity.
View Article and Find Full Text PDFCancers (Basel)
January 2025
Department of Neurosurgery, Institute of Science Tokyo Hospital, Tokyo 1130034, Japan.
: Resection of tumors invading the cavernous sinus (CS) carries a risk of injury to the cranial nerves and internal carotid artery. Therefore, radical surgery involving lesions around the CS remains challenging, especially for lesions invading the CS, optic sheath, and oculomotor cave. Here, we describe a surgical strategy for meningiomas invading these structures and report on the clinical outcomes.
View Article and Find Full Text PDFBMC Plant Biol
January 2025
PARC-Balochistan Agricultural Research and Development Center, Quetta, 87300, Pakistan.
Background: Grain number (GN) is one of the key yield contributing factors in modern wheat (Triticum aestivum) varieties. Fruiting efficiency (FE) is a key trait for increasing GN by making more spike assimilates available to reproductive structures. Thousand grain weight (TGW) is also an important component of grain yield.
View Article and Find Full Text PDFBMC Med Educ
January 2025
Department of Medical Education, Medical School, Jeonbuk National University, Jeonju, Republic of Korea.
Background: Cadaveric dissection is an essential practice for medical students to understand human anatomy and internalize professional attitudes toward death, such as empathy and respect for the deceased. Previous studies have focused on emotional responses such as shock, anxiety, and fear at the start of dissection practice. However, limited research exists on students' reflective thinking and perceptions before practice.
View Article and Find Full Text PDFScience
January 2025
Sex Chromosome Biology Laboratory, The Francis Crick Institute, London, UK.
The mammalian Y chromosome is essential for male fertility, but which Y genes regulate spermatogenesis is unresolved. We addressed this by generating 13 Y-deletant mouse models. In , , and deletants, spermatogenesis was impaired.
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