Seasonal heavy metal accumulations in the bivalve Barbatia decussate and their relationships with water quality and the metal-induced biochemical biomarkers.

Seasonal tissue concentrations of heavy metals, antioxidant enzymes, immunological components, and water quality parameters were investigated during 1 year in the ark clam, Barbatia decussate, from the coast of Lengeh port, located in the north of the Persian Gulf, Iran. The tissue accumulation of the heavy metals (Cd, Pb, Hg) significantly increased accumulations in late autumn and winter (P < 0.01). Theconcentrations of Ni and Cr nearly remained unchanged throughout the 1 year sampling period (P > 0.01). Seasonal changes were also observed in metal-induced biochemical components. In this regard, the malondialdehyde (MDA) levels and the activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) elevated throughout spring and summer and then declined during autumn and winter (P <  0.01). Phagocytosis activity significantly decreased from December to February and then increased from March to September (P < 0.01). Total hemocyte counts decreased from October to March and then elevated until April (P < 0.01). Significant relationships were found between tissue heavy metal concentrations, water quality parameters, and biochemical components (P < 0.01). The negative correlations were O vs. antioxidant enzymes, phagocytosis, and total counts of the hemocytes (THCs); pH vs. SOD; salinity vs. Cr; and temperature vs. GPx and Ni. Positive correlations were O vs. Cd, Pb, Hg, and Ni; temperature vs. phagocytosis and THCs; and turbidity vs. phagocytosis, THCs, CAT, and GPx. The results of the present study showed a seasonal pattern in the accumulation of heavy metals, with maximum levels in winter for the ark clam, B. decussate . Furthermore, antioxidant defense and immunity of B. decussate are reduced during winter, which may make B. decussate susceptible to diseases.