AI Article Synopsis
- Lacrimal fluid, or tears, holds potential as a source for noninvasive biomarkers, but the small sample volume often collected poses challenges for analysis.
- Researchers developed an effective pipeline for analyzing single tears from 23 healthy volunteers using ultrahigh-resolution proteomics, successfully identifying 890 proteins with reproducible quantification methods.
- The study found significant differences in protein profiles related to gender and time of day, indicating the feasibility of using tear analysis for personalized medicine approaches.
Article Abstract
Lacrimal fluid is an attractive source of noninvasive biomarkers, the main limitation being the small sample amounts typically collected. Advanced analytical methods to allow for proteomics profiling from a few microliters are needed to develop innovative biomarkers, with attractive perspectives of applications to precision medicine. This work describes an effective, analytical pipeline for single-tear analysis by ultrahigh-resolution, shotgun proteomics from 23 healthy human volunteers, leading to high-confidence identification of a total of 890 proteins. Highly reproducible quantification was achieved by either peak intensity, peak area, or spectral counting. Hierarchical clustering revealed a stratification of females vs. males that did not emerge from previous studies on pooled samples. Two subjects were monitored weekly over 3 weeks. The samples clustered by withdrawal time of day (morning vs. afternoon) but not by follow-up week, with elevated levels of components of the immune system in the morning samples. This study demonstrates feasibility of single-tear quantitative proteomics, envisaging contributions of this unconventional body fluid to individualized approaches in biomedicine.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8509675 | PMC |
| http://dx.doi.org/10.3390/ijms221910750 | DOI Listing |
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