AI Article Synopsis
- Adeno-associated viruses (AAV) are typically non-enveloped single-stranded DNA viruses, but they can form capsids within exosomes enhanced by the membrane associated accessory protein (MAAP).
- Researchers successfully produced homogeneous envelope-associated AAV (EA-AAV) capsids using a specific expression system and determined their structure through cryo-electron microscopy.
- The findings suggest that EA-AAV capsids share similar structural properties with non-envelope capsids, but they may offer potential therapeutic advantages such as better immune evasion and increased infectivity.
Article Abstract
Adeno-associated virus (AAV) are classified as non-enveloped ssDNA viruses. However, AAV capsids embedded within exosomes have been observed, and it has been suggested that the AAV membrane associated accessory protein (MAAP) may play a role in envelope-associated AAV (EA-AAV) capsid formation. Here, we observed and selected sufficient homogeneous EA-AAV capsids of AAV2, produced using the Sf9 baculoviral expression system, to determine the cryo-electron microscopy (cryo-EM) structure at 3.14 Å resolution. The reconstructed map confirmed that the EA-AAV capsid, showed no significant structural variation compared to the non-envelope capsid. In addition, the Sf9 expression system used implies the notion that MAAP may enhance exosome AAV encapsulation. Furthermore, we speculate that these EA-AAV capsids may have therapeutic benefits over the currently used non-envelope AAV capsids, with advantages in immune evasion and/or improved infectivity.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9911311 | PMC |
| http://dx.doi.org/10.1016/j.virol.2021.09.010 | DOI Listing |
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