Characterization of gene family and functional analysis of involved in heat tolerance in radish ( L.).

Unlabelled: Plant annexins are a kind of conserved Ca-dependent phospholipid-binding proteins which are involved in plant growth, development and stress tolerance. Radish is an economically important annual or biennial root vegetable crop worldwide. However, the genome-wide characterization of () gene family remain largely unexplored in radish. In this study, a comprehensive identification of a gene family was performed at the whole genome level in radish. In total, ten genes were identified, and these putative RsANN proteins shared typical characteristics of the annexin family proteins. Phylogenetic analysis showed that the RsANNs together with annexin from Arabidopsis and rice were clustered into five groups with shared similar motif patterns. Chromosomal localization showed that these ten genes were distributed on six chromosomes (R3-R8) of radish. Several -elements involved in abiotic stress response were identified in the promoter regions of genes. Expression profile analysis indicated that the genes exhibited tissue-specific patterns at different growth stages and tissues. The Real-time quantitative PCR (RT-qPCR) revealed that the expression of most genes was induced under various abiotic stresses including heat, drought, salinity, oxidization and ABA stress. In addition, stress assays showed that overexpression of improved plant's growth and heat tolerance, while artificial microRNAs (amiRNA)-mediated knockdown of caused dramatically decreased survival ratio of Arabidopsis plants. These findings not only demonstrate that might play a critical role in the heat stress response of radish, but also facilitate clarifying the molecular mechanism of genes in regulating the biological process governing plant growth and development.

Supplementary Information: The online version contains supplementary material available at 10.1007/s12298-021-01056-5.