Objectives - strategic objective - creation of fundamentally new inhibitors of metastasing of malignant tumors due to the radical surgeries carried out on them. Specific tactical objective - revealing the possible cytolytic and citostatic effectiveness of the drug "Amphicezine" created by us on atypical fibroblastic cells. The effect of "Amphicezine" on viable D60 p4 line cell count was measured using live cell imaging as a measure of cell proliferation. The results of "Amphicezine" treatment of D60 p4 cell line didn't shown a cytolytic/cytotoxic effect for dermal atypical fibroblast cells. Control cells and cells added 2.5% and 1% "Amphicezine" continued to proliferate, while cells treated with 5% and 10% "Amphicezine" didn't change cell count. The cell growth curves indicate viable cell count increase for control and 2.5%, 1% "Amphicezine" treated cells. 93.5% inhibition on D60 p4 were shown by 10% "Amphicezine" and 79.8% inhibition were shown on 5% "Amphicezine" treated cells. Cells treated with 2.5% and 1% "Amphicezine" showed similar proliferation profile as the control sample. The effect of "Amphicezine" on viable D60 p4 cell line cell count was measured using live cell imaging as a measure of cell proliferation. Maximal inhibition on D60 p4 cells were shown by 10% "Amphicezine" (93.5%). The results show that the growth curves for viable D60 p4 cell culture didn't indicate a cytolytic / cytotoxic effect for all samples (concentrations) treated with "Amphicezine". Cells treated with 2.5% and 1% "Amphicezine" showed similar proliferation profile as the control (PBS) sample.
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