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AgZDS, a gene encoding ζ-carotene desaturase, increases lutein and β-carotene contents in transgenic Arabidopsis and celery. | LitMetric

AgZDS, a gene encoding ζ-carotene desaturase, increases lutein and β-carotene contents in transgenic Arabidopsis and celery.

Plant Sci

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China, College of Horticulture, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China. Electronic address:

Published: November 2021

AI Article Synopsis

  • Z-carotene desaturase (ZDS) is a crucial enzyme for carotenoid biosynthesis, and this study focuses on cloning and overexpressing the AgZDS gene from celery to investigate its function in both Arabidopsis thaliana and celery.
  • The results showed that overexpressing AgZDS significantly increased lutein and β-carotene levels, with the highest concentrations found in specific transgenic lines, alongside up-regulation of several key carotenoid biosynthesis genes.
  • This research is the first to report on the role of a structural gene related to carotenoid biosynthesis in transgenic celery, suggesting potential benefits for improving celery quality through enhanced antioxidant and photosynthetic capacities.

Article Abstract

ζ-Carotene desaturase (ZDS) is one of the key enzymes regulating carotenoids biosynthesis and accumulation. Celery transgenic efficiency is low and it is difficult to obtain transgenic plants. The study on ZDS was limited in celery. Here, the AgZDS gene was cloned from celery and overexpressed in Arabidopsis thaliana and celery to verify its function. The AgZDS has typical characteristic of ZDS protein and is highly conserved in higher plants. Phylogenetic analysis showed that AgZDS has the closest evolutionary relationship with ZDSs from Solanum lycopersicum, Capsicum annuum and Tagetes erecta. Overexpression of AgZDS gene in A. thaliana and celery resulted in increased accumulations of lutein and β-carotene and up-regulated the expression levels of the genes involved in carotenoids biosynthesis. The contents of lutein and β-carotene in two lines, AtL1 and AgL5, were the highest in transgenic A. thaliana and celery, respectively. The relative expression levels of 5 genes (AtPDS, AtZISO, AtZEP, AtNCED3, and AtCCD4) were up-regulated compared to the wild type plants. The relative expression levels of most genes in carotenoids biosynthesis pathway, such as AgPDS, AgCRTISO1, and AgZISO, were up-regulated in transgenic celery plants. The antioxidant capacity of A. thaliana and photosynthetic capacity of celery were also enhanced. This research is the first report on the function of structure gene related to carotenoid biosynthesis in transgenic celery plants. The findings in this study demonstrated the roles of AgZDS in regulating carotenoids metabolism of celery, which laid a potential foundation for quality improvement of celery.

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Source
http://dx.doi.org/10.1016/j.plantsci.2021.111043DOI Listing

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