AI Article Synopsis
- The study introduces a new method for antifungal screening that enhances traditional broth dilution techniques by using time-resolved imaging to evaluate fungal growth.
- This method is effective for various filamentous fungi and is particularly useful when using spores for testing.
- It provides accurate results regarding fungal susceptibility to different compounds in just a few hours, while accommodating the unique growth characteristics of different fungal strains.
Article Abstract
Robust antifungal screening is technically challenging particularly for filamentous fungi. We present a method for undertaking antifungal screening assays that builds upon existing broth dilution protocols and incorporates time resolved image-based assessment of fungal growth. We show that the method performs with different fungi, particularly those for which spores can be used as inoculum, and with different compound classes, can accurately assess susceptibility or otherwise in only few hours and can even account for differences in inherent growth properties of strains.
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| http://dx.doi.org/10.1016/j.mimet.2021.106342 | DOI Listing |
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