Plasmonic metallic nanostructures with anisotropic design have unusual polarization-selective characteristic which can be utilized to build nanopolarizers at the nanoscale. Herein, we propose a dual-color image display platform by reconfiguring two types of silver nanoblocks in a single-celled metasurface. Governed by Malus's law, the two types of silver nanoblocks both acting as nanopolarizers with different orientations can continuously modulate the intensity of incident linearly polarized red and green light pixel-by-pixel, respectively. As a result, an ultra-compact, high-resolution, and continuous-greyscale dual-color image can be recorded right at the surface of the meta-device. We demonstrate the dual-color Malus metasurface by successfully encoding and decoding a red-green continuously-grayscale image into a metasurface sample. The experimentally captured meta-image with high-fidelity and resolution as high as 63500 dots per inch (dpi) has verified our proposal. With the advantages such as continuous grayscale modulation, ultrathin, high stability and high density, the proposed dual-color encoded metasurfaces can be readily used in ultra-compact image displays, high-end anti-counterfeiting, high-density optical information storage and information encryption, etc.
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http://dx.doi.org/10.1364/OE.433664 | DOI Listing |
Commun Eng
March 2025
Leibniz Institute of Photonic Technology, Albert-Einstein-Str. 9, 07745, Jena, Germany.
In biological imaging, there is a demand for cost-effective, high-resolution techniques to study dynamic intracellular processes. Structured illumination microscopy (SIM) is ideal for achieving high axial and lateral resolution in live samples due to its optical sectioning and low phototoxicity. However, conventional SIM systems remain expensive and complex.
View Article and Find Full Text PDFNAR Genom Bioinform
March 2025
Digital PCR Center (DIGPCR), Ghent University, 9820 Merelbeke, Belgium.
Digital polymerase chain reaction (dPCR) is a state-of-the-art targeted quantification method of nucleic acids. The technology is based on massive partitioning of a reaction mixture into individual PCR reactions. The resulting partition-level end-point fluorescence intensities are used to classify partitions as positive or negative, i.
View Article and Find Full Text PDFAngew Chem Int Ed Engl
March 2025
Chinese Academy of Sciences Dalian Institute of Chemical Physics, Department of Biotechnology, CHINA.
Fluorescence super-resolution microscopy has enabled nanoscale imaging of intracellular structures, but it remains challenging to simultaneously achieve structural imaging and quantitative functional characterization, such as pH measurement, within the same region. Here, we introduce Two-Color Single-Molecule Blinking Ratiometricity (2C-SMBR), a novel method that integrates structural and functional imaging with single-molecule precision. By loading lysosomes with two pH-dependent spontaneously blinking fluorophores of distinct colors, 2C-SMBR leverages single-molecule localization of either fluorophore to achieve nanoscale structural imaging of lysosomes, while the ratiometric analysis of blinking dynamics between the two fluorophores provides quantitative pH measurement at the single-lysosome level.
View Article and Find Full Text PDFNeuron
February 2025
Instituto Cajal CSIC, Madrid 28002, Spain. Electronic address:
Integrating analyses of genetically defined cell types with population-level approaches remains poorly explored. We investigated this question by focusing on hippocampal spatial maps and the contribution of two genetically defined pyramidal cell types in the deep and superficial CA1 sublayers. Using single- and dual-color miniscope imaging in mice running along a linear track, we found that population activity from these cells exhibited three-dimensional ring manifolds that encoded the animal position and running direction.
View Article and Find Full Text PDFAnal Chim Acta
March 2025
Anhui Innovative Center for Drug Basic Research of Metabolic Diseases, Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Wannan Medical College, Wuhu, 241002, PR China; School of Pharmacy, Wannan Medical College, Wuhu, 241002, PR China. Electronic address:
Background: Elaborating the subcellular polarity is pivotal for pathophysiological research and early disease diagnosis. Despite its significance, achieving simultaneous two-color fluorescence imaging and quantitative analysis of polarity across multiple organelles remains challenging. This limitation primarily stems from the lack of single fluorescent (SF) probes capable of such precise and multifaceted functionality.
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