The feedback control to optical tweezers is an obvious approach to improve the optical confinement. However, the electronic-based feedback controlling system in optical tweezers usually consists of complex software and hardware, and its performance is limited by the inevitable noise and time-delay from detecting and controlling devices. Here, we present and demonstrate the dual-beam intracavity optical tweezers enabling all-optical independent radial and axial self-feedback control of the trapped particle's radial and axial motions. We have achieved the highest optical confinement per unit intensity to date, to the best of our knowledge. Moreover, both the axial and radial confinements are adjustable in real-time, through tuning the foci offset of the clockwise and counter-clockwise beams. As a result, we realized three-dimensional self-feedback control of the trapped particle's motions with an equivalent level in the experiment. The dual-beam intracavity optical tweezers will significantly expand the range of optical manipulation in further studies of biology, physics and precise measurement, especially for the sample that is extremely sensitive to heat.
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http://dx.doi.org/10.1364/OE.431946 | DOI Listing |
Immunol Rev
December 2024
Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
αβT cells protect vertebrates against many diseases, optimizing surveillance using mechanical force to distinguish between pathophysiologic cellular alterations and normal self-constituents. The multi-subunit αβT-cell receptor (TCR) operates outside of thermal equilibrium, harvesting energy via physical forces generated by T-cell motility and actin-myosin machinery. When a peptide-bound major histocompatibility complex molecule (pMHC) on an antigen presenting cell is ligated, the αβTCR on the T cell leverages force to form a catch bond, prolonging bond lifetime, and enhancing antigen discrimination.
View Article and Find Full Text PDFSubcell Biochem
December 2024
Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM), and Department of Molecular Biology, Universidad Autónoma de Madrid, Madrid, Spain.
Structural biology techniques have greatly contributed to unveiling the interplay between molecular structure, physico-chemical properties, and biological function of viruses. In recent years, classic structural approaches are being complemented by single-molecule techniques such as atomic force microscopy and optical tweezers to study physical features of viral particles that are not accessible to classic structural techniques. Among these features are mechanical properties such as stiffness, intrinsic elasticity, tensile strength, and material fatigue.
View Article and Find Full Text PDFSubcell Biochem
December 2024
Centro de Tecnologías Físicas, Universitat Politècnica de València, Valencia, Spain.
A virus is a complex molecular machine that propagates by channeling its genetic information from cell to cell. Unlike macroscopic engines, it operates in a nanoscopic world under continuous thermal agitation. Viruses have developed efficient passive and active strategies to pack and release nucleic acids.
View Article and Find Full Text PDFBiomech Model Mechanobiol
December 2024
Department of Biomedical Engineering, Virginia Commonwealth University, 401 W. Main St., Richmond, VA, 23284, USA.
Embryonic development, wound healing, and organogenesis all require assembly of the extracellular matrix protein fibronectin (FN) into insoluble, viscoelastic fibrils. FN fibrils mediate cell migration, force generation, angiogenic sprouting, and collagen deposition. While the critical role of FN fibrils has long been appreciated, we still have an extremely poor understanding of their mechanical properties and how these mechanical properties facilitate cellular responses.
View Article and Find Full Text PDFIn this Letter, we have proposed an all-optical scheme for chiral particle separation with a microcylinder-pair system (MCPS) with a micrometer scale channel, applicable in microfluidic environments. By illuminating the MCPS with two counter-incident plane waves of orthogonal polarization, the electromagnetic chirality gradient can be generated. The MCPS can also enhance chirality-dependent lateral optical forces of the coupled fields so that the setup can shift trapping equilibrium positions for opposite-handedness nanoparticles and make the sideways motion observable.
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