Urinary level of heavy metals in people working in smoking cafés.

Previous researches have reported significant levels of heavy metals in indoor air of smoking cafés. The current research aimed to evaluate the potential exposure of smoking cafés workers to heavy metals through quantifying amounts of these pollutants in urine samples. To this end, 35 waterpipe/cigarette cafés workers were selected as the exposed population, 35 employees from non-smoking cafe' as the control group 1 (CG1) and 25 cases of the normal citizens as the control group 2 (CG2); and their urine samples were taken. Samples were then prepared by acid digestion approach and measured by the ICP in order to determine amount of heavy metals in them. The findings of the current work show the significant difference among urinary levels of metals in the exposed and both CG1 and CG2 (P value < 0.05). Furthermore, urinary metal levels in samples collected from smoking café workers after the exposure were considerably higher than those were collected before the exposure (P-value <0.05). According to the findings, "tobacco type" could be considered as a leading factor for heavy metal exposure in studied smokers. Additionally, a positive and significant association was found between urinary metals and urinary levels of 8-OHdG (a markers of DNA degradation through oxidative stress). Therefore, workers in waterpipe/cigarette café can be classified as high risk people in terms of DNA oxidative damage.