Functional analysis of co-expression networks of zebrafish reveals enrichment of pathways associated with development and disease.

Human Angiotensin I Converting Enzyme 2 (ACE2) plays an essential role in blood pressure regulation and SARS-CoV-2 entry. ACE2 has a highly conserved, one-to-one ortholog () in zebrafish, which is an important model for human diseases. However, the zebrafish expression profile has not yet been studied during early development, between genders, across different genotypes, or in disease. Moreover, a network-based meta-analysis for the extraction of functionally enriched pathways associated with differential expression is lacking in the literature. Herein, we first identified significant development-, tissue-, genotype-, and gender-specific modulations in expression via meta-analysis of zebrafish Affymetrix transcriptomics datasets ( = 107); and the correlation analysis of meta-differential expression profile revealed distinct positively and negatively correlated local functionally enriched gene networks. Moreover, we demonstrated that expression was significantly modulated under different physiological and pathological conditions related to development, tissue, gender, diet, infection, and inflammation using additional RNA-seq datasets. Our findings implicate a novel translational role for zebrafish in organ differentiation and pathologies observed in the intestines and liver.