AI Article Synopsis
- There is a critical need for quick, affordable, and easy-to-use diagnostic methods for tuberculosis (TB) at the point-of-care.
- Researchers developed a dye conjugate, 3HC-3-Tre, which can specifically detect Mycobacterium tuberculosis (Mtb) in just minutes.
- This new method showcases a significant increase in fluorescence for detecting Mtb, allowing for rapid and effective visualization that could enhance TB diagnosis in resource-limited environments.
Article Abstract
There is an urgent need for point-of-care tuberculosis (TB) diagnostic methods that are fast, inexpensive, and operationally simple. Here, we report on a bright solvatochromic dye trehalose conjugate that specifically detects (Mtb) in minutes. 3-Hydroxychromone (3HC) dyes, known for having high fluorescence quantum yields, exhibit shifts in fluorescence intensity in response to changes in environmental polarity. We synthesized two analogs of 3HC-trehalose conjugates (3HC-2-Tre and 3HC-3-Tre) and determined that 3HC-3-Tre has exceptionally favorable properties for Mtb detection. 3HC-3-Tre-labeled mycobacterial cells displayed a 10-fold increase in fluorescence intensity compared to our previous reports on the dye 4,4--dimethylaminonapthalimide (DMN-Tre). Excitingly, we detected fluorescent Mtb cells within 10 min of probe treatment. Thus, 3HC-3-Tre permits rapid visualization of mycobacteria that ultimately could empower improved Mtb detection at the point-of-care in low-resource settings.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8479770 | PMC |
| http://dx.doi.org/10.1021/jacsau.1c00173 | DOI Listing |
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