AI Article Synopsis
- The study used qRT-PCR to assess the expression of a specific gene and various miRNAs in thyroid tumors and nearby noncancerous tissues, as well as to check the methylation status of the gene's promoter.
- There was a significant decrease in the gene's expression in tumors compared to noncancerous tissues, while the levels of miR-182, miR-183, and miR-375 were higher in the tumors.
- The findings suggest that the gene plays a role in the development of papillary thyroid carcinoma and that its regulation is influenced more by miRNAs than by promoter methylation.
Article Abstract
Methods: In this study, qRT-PCR was used to investigate the expression levels of the gene and of miR-182, miR-183, miR-375, and miR-96 in thyroid tumors and adjacent noncancerous tissues. We also investigated the methylation status of the promoter by methylation-specific PCR in tumors and adjacent noncancerous tissues.
Results: We observed a statistically significant downregulation of expression in tumors compared to noncancerous tissue samples. The methylation levels of tumors and matched noncancerous tissues were similar, but miR-182, miR-183, and miR-375 expression levels were elevated in tumor tissues compared to noncancerous tissue samples.
Conclusions: Our results indicate that gene expression is associated with the pathogenesis of papillary thyroid carcinoma (PTC), and the epigenetic control of the gene is regulated by miRNAs rather than by promoter methylation.
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8486500 | PMC |
| http://dx.doi.org/10.1155/2021/1588220 | DOI Listing |
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