In this study, the effect and effect mechanisms of [Pyr1]apelin-13, the dominant apelin isoform in the human cardiovascular tissues and human plasma, on vascular contractility were investigated. The vascular rings obtained from the thoracic aortas of the male Wistar Albino rats were placed in the isolated tissue bath system. After the equilibration period, [Pyr1]apelin-13 (10-9 to 10-6 M) was applied cumulatively to the aortic rings pre-contracted with phenylephrine in the plateau phase. The protocol was repeated in the presence of specific signaling pathway inhibitors (F13A, L-NAME, dorsomorphin, TEA, U0126, or indomethacin) to determine the effect mechanisms of [Pyr1]apelin-13. [Pyr1]apelin-13 induced a dose-dependent relaxation in the pre-contracted aortic rings. APJ, eNOS, AMPK, and potassium channel inhibition statistically significantly decreased the vasodilator effect of [Pyr1]apelin-13. MAPK and COX inhibition didn't statistically significantly changed the vasodilator effect of [Pyr1]apelin-13. In conclusion, [Pyr1]apelin-13 relaxes the rat thoracic aorta via APJ, NO, AMPK, and potassium channels.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.4149/gpb_20210258 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
New Application of an Old Drug: Anti-Diabetic Properties of Phloroglucinol.
Int J Mol Sci
September 2024
Clinical Research Support Centre, Medical University of Bialystok, 15-089 Bialystok, Poland.
Phloroglucinol (PHG), an analgesic and spasmolytic drug, shows promise in preventing high-fat-diet (HFD)-induced non-alcoholic fatty liver disease (NAFLD) and insulin resistance. In Wistar rats, 10 weeks of PHG treatment did not prevent HFD-induced weight gain but significantly mitigated fasting hyperglycemia, impaired insulin responses, and liver steatosis. This protective effect was not linked to hepatic lipogenesis or AMP-activated protein kinase (AMPK) activation.
View Article and Find Full Text PDFThe multifaceted role of different SnRK gene family members in regulating multiple abiotic stresses in plants.
Physiol Plant
October 2024
Division of Crop Physiology and Biochemistry, ICAR-National Rice Research Institute, Cuttack, Odisha, India.
Abiotic stresses are a major constraint for agricultural productivity and food security in today's era of climate change. Plants can experience different types of abiotic stresses, either individually or in combination. Sometimes, more than one stress event may occur simultaneously or one after another during the lifecycle of the plant.
View Article and Find Full Text PDFThe thienopyridine A-769662 and benzimidazole 991 inhibit human TASK-3 potassium channels in an AMPK-independent manner.
Biochem Pharmacol
December 2024
Centre for Discovery Brain Sciences, Hugh Robson Building, University of Edinburgh, Edinburgh EH8 9XD, UK. Electronic address:
Article Synopsis
- TASK-1/3 channels in carotid body type 1 cells are key for sensing low oxygen levels, affecting potassium currents and ultimately influencing breathing patterns.
- Recent studies questioned whether AMP-activated protein kinase (AMPK) directly inhibits TASK-3 channels in response to hypoxia, although a recognizable phosphorylation motif was found in human TASK-3.
- Experiments with various AMPK activators indicated that two compounds, A-769662 and 991, inhibit hTASK-3 currents, while establishing that AMPK does not regulate TASK-3 currents as initially proposed.
Acacetin is a Promising Drug Candidate for Cardiovascular Diseases.
Am J Chin Med
November 2024
Nanjing Amazigh Pharma Limited, Nanjing, Jiangsu 210032, P. R. China.
Article Synopsis
- * Acacetin inhibits various potassium currents, which can help suppress experimental J-wave syndromes and offers protective effects against conditions like ischemia/reperfusion injury and atherosclerosis.
- * The review emphasizes acacetin's role as a promising drug candidate for cardiovascular diseases by enhancing cellular signaling pathways that combat oxidation, inflammation, and cell death.
Unconjugated bilirubin promotes uric acid restoration by activating hepatic AMPK pathway.
Free Radic Biol Med
November 2024
Department of Pharmacology, School of Basic Medical Sciences, Zhejiang University, Hangzhou, 310058, China; Key Laboratory of Clinical Cancer Pharmacology and Toxicology Research of Zhejiang Province, Hangzhou First People's Hospital, Hangzhou, 310006, China; Westlake Laboratory of Life Sciences and Biomedicine of Zhejiang Province, Hangzhou, 310024, China. Electronic address:
Article Synopsis
- Hyperuricemia, a condition leading to gout, is often caused by high activity of xanthine oxidase, which produces uric acid.
- This study found that lower bilirubin levels are linked to higher uric acid levels in patients with new-onset hyperuricemia and advanced gout.
- Researchers showed that bilirubin could reduce uric acid levels in mice by enhancing liver function and cellular processes, suggesting it might offer a new approach for managing hyperuricemia in humans.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!