Fed-batch simultaneous saccharification and fermentation including in-situ recovery for enhanced butanol production from rice straw.

This paper describes a study of fed-batch SSFR (simultaneous saccharification, fermentation and recovery) for butanol production from alkaline-pretreated rice straw (RS) in a 2-L stirred tank reactor. The initial solid (9.2% w/v) and enzyme (19.9 FPU g-dw) loadings were previously optimized by 50-mL batch SSF assays. Maximum butanol concentration of 24.80 g L was obtained after three biomass feedings that doubled the RS load (18.4% w/v). Butanol productivity (0.344 g Lh) also increased two-fold in comparison with batch SSF without recovery (0.170 g Lh). Although fed-batch SSFR was able to operate with a single initial enzyme dosage, an extra dosage of nutrients was required with the biomass additions to achieve this high productivity. The study showed that SSFR can efficiently improve butanol production from a lignocellulosic biomass accompanied by the efficient use of the enzyme.