Bio-Layer Interferometry (BLI) enables the detection and characterization of molecular interactions in real-time without the hassle and interference of labeling. The affinity constant (K) obtained in the BLI analysis is an excellent indicator of quality of biomolecules such as antibodies, aptamers, peptides, etc. This method was used to analyze a peptide macrocycle against the Abrax protein, but can be used for any peptide macrocycle/analyte system.
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