gen. nov., sp. nov., a new member of the order , isolated from a freshwater aquarium.

A novel bacterium, strain SJAQ100, was isolated from a freshwater aquarium and was characterized taxonomically and phylogenetically. Strain SJAQ100 was a Gram-stain-negative, aerobic, rod-shaped and non-motile bacterium. The strain grew optimally with 0 % NaCl and at 25-37 °C on Reasoner's 2A agar. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that the strain SJAQ100 clustered with members of in the order , but sequence similarities to known species were less than 96.5 %. The genomic DNA G+C content of strain SJAQ100 was 71.2 mol%. Genomic comparisons of strain SJAQ100 with species in the order were made using the Genome-to-Genome Distance Calculator, average nucleotide identity and average amino acid identity analyses (values indicated ≤22.1, ≤78.1, and ≤68.1 % respectively). Strain SJAQ100 contained C and C 7/C 6 as major fatty acids and Q-8 as the major quinone. The major polyamines were putrescine and cadaverine. Strain SJAQ100 contained phosphatidylethanolamine and diphosphatidylglycerol as major polar lipids. Based on the genotypic, chemotaxonomic and phenotypic results, strain SJAQ100 represents a novel genus and species, gen. nov., sp. nov., which belongs to order and the class . The type strain is SJAQ100 (=KCTC 72203=CGMCC 1.18869=MCC 4385).