Xyloglucan is one of the main components of the primary cell wall in most species of plants. This protocol describes a method to analyze the composition of the enzyme-accessible and enzyme-inaccessible fractions of xyloglucan in the model species . It is based on digestion with an endoglucanase that attacks unsubstituted glucose residues in the backbone. The identities and relative amounts of released xyloglucan fragments are then determined using MALDI-TOF mass spectrometry.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438494 | PMC |
| http://dx.doi.org/10.21769/BioProtoc.2569 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The transcription factor SlLBD40 regulates seed germination by inhibiting cell wall remodeling enzymes during endosperm weakening.
Plant Physiol
January 2025
College of Horticulture, China Agricultural University, Beijing 100193, China.
Uniform seed germination is crucial for consistent seedling emergence and efficient seedling production. In this study, we identified a seed-expressed protein in tomato (Solanum lycopersicum), lateral organ boundaries domain 40 (SlLBD40), that regulates germination speed. CRISPR/Cas9-generated SlLBD40 knockout mutants exhibited faster germination due to enhanced seed imbibition, independent of the seed coat.
View Article and Find Full Text PDFGenome-Wide Identification of Xyloglucan Endotransglucosylase/Hydrolase Multigene Family in Chinese Jujube () and Their Expression Patterns Under Different Environmental Stresses.
Plants (Basel)
December 2024
State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
The Xyloglucan endotransglucosylase/hydrolase (XTH) family, a group of cell wall-modifying enzymes, plays crucial roles in plant growth, development, and stress adaptation. The quality and yield of Chinese jujube () fruit are significantly impacted by environmental stresses, including excessive salinity, drought, freezing, and disease. However, there has been no report of the XTH encoding genes present in the Chinese jujube genome and their response transcription level under various stresses.
View Article and Find Full Text PDFGreen Synthesis of Urethane-Linked Tamarind Seed Xyloglucan: Thermal Stability, Antibacterial Properties, and DFT Study.
Biopolymers
January 2025
Post Graduate & Research Department of Chemistry, Mahatma Gandhi College, Thiruvananthapuram, India.
This study presents a feasible, one-pot synthesis approach for the preparation of a composite biopolymer material derived from tamarind seed xyloglucan (XG) by utilizing isocyanate chemistry. Through a facile reaction process, urethane bonds are formed in XG, resulting in the formation of a crosslinked network. FTIR spectra confirm the successful urethane link formation in XG via the OH-NCO reaction, and CHN analysis provides insights into the elemental composition.
View Article and Find Full Text PDFMutational study of a lytic polysaccharide monooxygenase from Myceliophthora thermophila (MtLPMO9F): Structural insights into substrate specificity and regioselectivity.
Int J Biol Macromol
December 2024
Laboratory of Structural Biology and Biotechnology, Department of Chemical Engineering University of Patras, Patras, Greece. Electronic address:
Lytic polysaccharide monooxygenases (LPMOs) are key enzymes for the biotechnological exploitation of lignocellulosic biomass, yet their efficient application depends on the in-depth understanding of their mechanism of action. Here, we describe the structural and mutational characterization of a C4-active LPMO from Myceliophthora thermophila, MtLPMO9F, that belongs to auxiliary activity family 9 (AA9). MtLPMO9F is active on cellulose, cello-oligosaccharides and xyloglucan.
View Article and Find Full Text PDFIn Planta Ectopic Expression of Two Sub-types of Tomato Cellulose Synthase-like M (CslM) Genes Affects Cell Wall Integrity and Supports a Role in Arabinogalactan and/or Rhamnogalacturonan-I Biosynthesis.
Plant Cell Physiol
December 2024
School of Agriculture, Food and Wine, University of Adelaide, PMB1, Glen Osmond, 5064, Australia.
Diversification of the cellulose synthase superfamily of glycosyltransferases has provided plants with the ability to synthesise varied cell wall polysaccharides such as xyloglucan, mannans and the mixed-linkage glucans of cereals. Surprisingly, some but not all members of the cellulose synthase-like M (CslM) gene family have recently been shown to be involved in the glycosylation of the aglycone core of a range of triterpenoid saponins. However, no cell wall activity has yet been attributed to any of the CslM gene family members.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!