AI Article Synopsis
- - The study evaluates the effectiveness of the PowerChek Multiplex Real-time PCR Kit in detecting SARS-CoV-2, influenza A & B, and RSV simultaneously, compared to another test called BioFire Respiratory Panel 2.1 (RP2.1).
- - Results showed a perfect agreement of 100% for detecting SARS-CoV-2 and influenza viruses, with slightly lower but still high accuracy for RSV detection.
- - The assay shows promising sensitivity and accuracy, indicating its potential as a useful tool for diagnosing infections when these viruses are circulating together in healthcare settings.
Article Abstract
The potential co-circulation of SARS-CoV-2, influenza, and respiratory syncytial virus (RSV) could pose an unprecedented challenge to healthcare systems worldwide. Here, we compared the performance of the PowerChek SARS-CoV-2, Influenza A&B, RSV Multiplex Real-time PCR Kit (PowerChek) for simultaneous detection of SARS-CoV-2, influenza A and B, and respiratory syncytial virus with that of BioFire Respiratory Panel 2.1 (RP2.1) using 175 nasopharyngeal swab (NPS) specimens. Positive percent agreement and negative percent agreement of the PowerChek assay compared to RP2.1 were as follows: 100 % (40/40) and 100 % (135/135) for SARS-CoV-2; 100 % (39/39) and 100 % (136/136) for influenza A; 100 % (35/35) and 100 % (140/140) for influenza B; and 93.1 % (27/29) and 100 % (146/146) for RSV, respectively. The limit of detection (LOD) was accessed using RNA standards for each virus, and the LOD values of the PowerChek assay for SARS-CoV-2, influenza A and B, and RSV were 0.36, 1.24, 0.09, and 0.63 copies/μL, respectively. Our results demonstrate that the PowerChek assay is sensitive and accurate for detection of SARS-CoV-2, influenza A and B, and RSV, suggesting that this assay can be a valuable diagnostic tool when SARS-CoV-2, influenza, and RSV are co-circulating.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8482523 | PMC |
| http://dx.doi.org/10.1016/j.jviromet.2021.114304 | DOI Listing |
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