AI Article Synopsis
- - Prime editing is a technique that allows scientists to make precise changes to DNA, including deletions, insertions, and transversions, in various organisms, including mammals.
- - Researchers created a mouse model with a cataract disorder by using prime editor 3 (PE3) plasmids to induce targeted single-base deletions, achieving a notable deletion rate of 38.2%.
- - The study demonstrated that these genetic alterations could be successfully passed on to future generations, resulting in offspring that also exhibit cataract symptoms, highlighting the potential of prime editing in modeling genetic diseases.
Article Abstract
Prime editing enables efficient introduction of targeted transversions, insertions, and deletions in mammalian cells and several organisms. However, genetic disease models with base deletions by prime editing have not yet been reported in mice. Here, we successfully generate a mouse model with a cataract disorder through microinjection of prime editor 3 (PE3) plasmids to efficiently induce targeted single-base deletion. Notably, a generated mouse with a high G-deletion rate (38.2%) displays a nuclear cataract phenotype; the PE3-induced deletions in mutant mice achieve high rates of germline transmission to their progenies, with phenotypic inheritance of cataract. Our data propose that modeling a genetic disease with a single nucleotide deletion in mice can be achieved with prime genome editing .
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8463286 | PMC |
| http://dx.doi.org/10.1016/j.omtn.2021.06.020 | DOI Listing |
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