Efficacy of cyclic lipopeptides obtained from to inhibit the growth of isolated from cats.

Background And Aim: () is a dermatophyte fungal pathogen that causes ringworms. Cats are considered to be a dominant reservoir host enabling transmission to humans. The concerns of dermatophyte resistance were raised among the usage of antifungal drugs to treat the ringworm. This study aimed to evaluate the fungal activity of cyclic lipopeptides (CLPs) obtained from () as an alternative method for the inhibition of growth.

Materials And Methods: The culture plate of from confirmed cats with ringworm infection was provided. The purification of CLP extract, fengycin, iturin A, and surfactin was carried out from by preparative thin-layer chromatography (PTLC) coupled with solid-phase extraction (SPE) methods. Half-maximal effective concentration (EC) and agar well diffusion assays were performed to determine the efficacy of CLP extract, fengycin, iturin A, and surfactin to inhibit the growth of isolated from cats.

Results: All purified substances displayed antifungal activity to control the growth of when compared with 80% ethanol (control). EC values for CLP extract, fengycin, iturin A, and surfactin were 0.23, 0.05, 0.17, and 0.08 mg/mL, respectively. In agar well diffusion assay, the ability of CLP extract, fengycin, iturin A, and surfactin on fungal inhibition had no statistically significant difference at 24 and 48 h after treatment (p < 0.05). However, CLP extract showed a statistically significant difference on inhibition at 62.21% followed by surfactin with 59.04% at 72 h after treatment.

Conclusion: , CLPs revealed an inhibitory effect on growth which is a zoonotic pathogen that causes ringworms. This study suggests an alternative approach to control the growth of using substances obtained from as a biomedicine agent with antifungal activity.