AI Article Synopsis
- The rise of advanced high-resolution imaging technologies increases reliance on the assumption that cell structures remain intact during experiments.
- Current methods for checking cellular structure integrity, like electron cryo-tomography, are expensive, complex, and time-consuming.
- We developed the Rapid Cell Integrity Assessment (RCIA) assay, a simple and cost-effective method using light microscopy to quickly evaluate how experimental protocols affect cellular structures at the nanoscale.
Article Abstract
With the rapid increase and accessibility of high-resolution imaging technologies of cells, the interpretation of results relies more and more on the assumption that the three-dimensional integrity of the surrounding cellular landscape is not compromised by the experimental setup. However, the only available technology for directly probing the structural integrity of whole-cell preparations at the nanoscale is electron cryo-tomography, which is time-consuming, costly, and complex. We devised an accessible, inexpensive and reliable screening assay to quickly report on the compatibility of experimental protocols with preserving the structural integrity of whole-cell preparations at the nanoscale. Our Rapid Cell Integrity Assessment (RCIA) assay is executed at room temperature and relies solely on light microscopy imaging. Using cellular electron cryo-tomography as a benchmark, we verify that RCIA accurately unveils the adverse impact of reagents and/or protocols such as those used for virus inactivation or to arrest dynamic processes on the cellular nanoarchitecture.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8665072 | PMC |
| http://dx.doi.org/10.1016/j.jsb.2021.107801 | DOI Listing |
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