AI Article Synopsis
- The study investigates the role of porcine interferon (PoIFN) as a model for understanding interferon evolution in pigs, particularly focusing on the newly identified subtype IFN-αω found in domestic pigs.
- Researchers cloned the cDNA of PoIFN-αω from Chinese Bama miniature pigs, analyzed its tissue expression, and successfully produced a recombinant version of the protein in E. coli for further testing.
- The recombinant PoIFN-αω demonstrated antiviral effects against several pig viruses, showing promise as a treatment for viral infections, although its effectiveness varied depending on the virus and the timing of administration.
Article Abstract
Porcine interferon (PoIFN) complex represents an ideal model for studying IFN evolution that resulted from viral pressure during domestication. IFN-αω is an emergent subtype of type I IFNs which has been primarily characterized in domestic pigs. In this study, the PoIFN-αω cDNA was cloned from Chinese Bama miniature pigs by RT-PCR, and its tissue expression profile was analyzed by real-time RT-PCR. The cDNA was expressed in Escherichia coli as a His-tagged protein and purified by nickel affinity chromatography. The antiviral activities of recombinant PoIFN-αω (rPoIFN-αω) against four different pig viruses were measured using cytopathic effect (CPE) inhibition assay. Although the PoIFN-αω sequence of Bama miniature pigs was identical to that of domestic pigs, the tissue expression profiles differed significantly between the two pig species. The rPoIFN-αω showed dose-dependent pre-infection antiviral activities against porcine pseudorabies virus, vesicular stomatitis virus and porcine reproductive and respiratory syndrome virus, but not against porcine circovirus type 2. When used as treatment post infection with the three viruses, rPoIFN-αω showed the efficacy in decreasing CPE in the infected cells in a time-dependent manner. Therefore, the expressed rPoIFN-αω could be used as an antiviral agent against pig virus infections.
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| http://dx.doi.org/10.1007/s11259-021-09829-9 | DOI Listing |
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