AI Article Synopsis
- Mesenchymal stem cells (MSCs) show great promise in treating various diseases but face challenges with low survival rates post-transplantation.
- Researchers created multicellular spheroids from MSCs, which were injected into mice and were found to survive better and accumulate in the lungs compared to individual cells.
- The spheroids also effectively reduced inflammatory markers, suggesting that this new method enhances both the delivery and therapeutic effects of MSCs in treating lung-related inflammation.
Article Abstract
Background: Because of the excellent therapeutic potential, mesenchymal stem cells (MSCs) have been used as cell therapeutics for various diseases. However, the survival rate and duration of MSCs after transplantation are extremely low and short, respectively. To solve these problems, in this study, we prepared multicellular spheroids of MSCs and investigated their survival and function after intravenous injection in mice.
Methods And Results: The murine adipose-derived MSC line m17.ASC was cultured in agarose-based microwell plates to obtain size-controlled m17.ASC spheroids of an average diameter and cell number of approximately 170 μm and 1100 cells/spheroid, respectively. The intravenously injected m17.ASC spheroids mainly accumulated in the lung and showed a higher survival rate than suspended m17.ASC cells during the experimental period of 7 days. m17.ASC spheroids efficiently reduced the lipopolysaccharide-induced increase in plasma concentrations of interleukin-6 and tumor necrosis factor-α.
Conclusions: These results indicate that spheroid formation improved the pulmonary delivery and survival of MSCs, as well as their therapeutic potential against inflammatory pulmonary diseases.
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| http://dx.doi.org/10.1002/biot.202100137 | DOI Listing |
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