sp. strain EGB is a Gram-negative myxobacteria isolated from saline soil, and has considerable potential for the biocontrol of phytopathogenic fungi. However, the detailed mechanisms related to development and predatory behavior are unclear. To obtain a comprehensive overview of genetic features, the genome of strain EGB was sequenced, annotated, and compared with 10 other species. The strain EGB genome was assembled as a single circular chromosome of 9.4 Mb with 7916 coding genes. Phylogenomics analysis showed that strain EGB was most closely related to AB047A, and it was inferred to be a novel species within the genus. Comparative genomic analysis revealed that the pan-genome of genus was large and open. Only a small proportion of genes were specific to strain EGB, and most of them were annotated as hypothetical proteins. Subsequent analyses showed that strain EGB produced abundant extracellular enzymes such as chitinases and β-(1,3)-glucanases, and proteases to degrade the cell-wall components of phytopathogenic fungi. In addition, 35 biosynthetic gene clusters potentially coding for antimicrobial compounds were identified in the strain EGB, and the majority of them were present in the dispensable pan-genome with unexplored metabolites. Other genes related to secretion and regulation were also explored for strain EGB. This study opens new perspectives in the greater understanding of the predatory behavior of strain EGB, and facilitates a potential application in the biocontrol of fungal plant diseases in the future.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466578 | PMC |
| http://dx.doi.org/10.3390/genes12091421 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Degradation mechanism of difructose dianhydride III in Blautia species.
Appl Microbiol Biotechnol
November 2024
Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima, Kagoshima, 890-0065, Japan.
Di-fructofuranose 1,2':2,3' dianhydride (DFA-III) is a cyclic fructo-disaccharide, which is produced by the condensation of two fructose molecules via the caramelization or enzymatic reaction of inulin fructotransferase. A strain of Blautia producta was known to utilize DFA-III as a carbohydrate source; however, the mechanisms remain unclear. In this study, we characterized the glycoside hydrolase (GH) family 91 DFA-III hydrolase (DFA-IIIase) from B.
View Article and Find Full Text PDFExploring the Impact of Elements on the Reactivity of a Straightforward Procedure for Generating Vinyl-Carbazole Derivatives via a Frustrated Lewis Pair Mechanism.
ACS Omega
July 2024
Department of Applied Chemistry, National Chiayi University, Chiayi 60004, Taiwan.
Article Synopsis
- The study investigates how different chemical elements (Group 13 and Group 15) affect the reactivity of a carbazolation reaction involving phenylacetylene using theoretical methods like density functional theory.
- The research finds that the size of the atomic radius of the catalysts can impact the reaction’s efficiency, as smaller or larger atoms hinder proper orbital overlap, increasing activation energy and slowing the reaction.
- Among the Lewis acids tested, all except Tl(CF) were found to effectively catalyze the reaction, and only N-carbazole was expected to participate in the reaction when catalyzed by B(CF), supporting Hammond's postulate which suggests that earlier transition states lead to lower activation energy.
Detection of the antibiotic resistance genes content of intestinal Bacteroides, Parabacteroides and Phocaeicola isolates from healthy and carbapenem-treated patients from European countries.
BMC Microbiol
June 2024
Institute of Medical Microbiology, Albert Szent-Györgyi Health Centre and Medical School, University of Szeged, Szeged, Hungary.
Article Synopsis
- The study focuses on the Bacteroides fragilis group (BFG), which are significant anaerobic pathogens known for their high antibiotic resistance, and aims to compare the antibiotic resistance genes in healthy individuals' microbiota with those from infected patients.
- Researchers found 13 antibiotic resistance determinants in 184 intestinal BFG strains collected from five European countries, revealing differences in gene prevalence compared to previous studies, particularly in mobile antibiotic resistance genes.
- The findings suggest novel characteristics in intestinal Bacteroides strains, indicating differing prevalence of resistance genes based on their microbiota composition and offering insights into their resistance mechanisms.
Pediococcus pentosaceus ZZ61 enhances growth performance and pathogenic resistance of silkworm Bombyx mori by regulating gut microbiota and metabolites.
Bioresour Technol
June 2024
State Key Laboratory of Resource Insects, College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing 400715, China. Electronic address:
Probiotics have attracted considerable attention in animal husbandry due to their positive effect on animal growth and health. This study aimed to screen candidate probiotic strain promoting the growth and health of silkworm and reveal the potential mechanisms. A novel probiotic Pediococcus pentosaceus strain (ZZ61) substantially promoted body weight gain, feed efficiency, and silk yield.
View Article and Find Full Text PDFsymbiosis with louse flies: multiple origins, coevolutionary dynamics, and metabolic significance.
mSystems
October 2023
Department of Parasitology, Faculty of Science, University of South Bohemia, České Budějovice, Czechia.
Insects that live exclusively on vertebrate blood utilize symbiotic bacteria as a source of essential compounds, e.g., B vitamins.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!