Biofilm Producing Isolates from Vaginal Microbiota.

Background: is an important cause of infection in the hospital as well as in the community.

Methods: A prospective study was done in Medical College, Kolkata for a period of 2 years (from January 2018 to December 2019). After obtaining clearance from the Institutional Ethics Committee, isolates from cases of vaginitis were included in the study. Identification of species was done by Gram stain and conventional biochemical tests along with automated identification by VITEK 2 Compact. These isolates were tested for antimicrobial susceptibility to different antibiotics by Kirby Bauer disc diffusion method and minimum inhibitory concentration (MIC) by VITEK 2 Compact. Interpretation of susceptibility was done according to the Clinical and Laboratory Standards Institute (CLSI) 2017 guidelines. Biofilm detection for species was done.

Results: During the period of 2 years, 39 isolates of spp. were obtained from vaginitis cases. Among these, 27 were and 12 . All isolates were highly susceptible to vancomycin, teicoplanin, and linezolid. Biofilm was detected in eight isolates of which five were strong biofilm producer and three moderate biofilm producers.

Conclusion: Biofilm production is an important virulence factor in isolates from vaginitis.