AI Article Synopsis
- The study found that removing the Labile Iron Pool (LIP) in macrophage cells increases oxidation levels, indicating that LIP normally reacts with peroxynitrite to reduce its oxidants.
- This discovery shifts the understanding of LIP's role in biochemistry and prompts further questions on its interaction with peroxynitrite.
- The follow-up research characterized this interaction, revealing it functions as a catalytic system with a specific reaction rate, suggesting LIP acts as a constant peroxynitrite reductase in these cells.
Article Abstract
While investigating peroxynitrite-dependent oxidation in murine RAW 264.7 macrophage cells, we observed that removal of the Labile Iron Pool (LIP) by chelation increases the intracellular oxidation of the fluorescent indicator HDCF, so we concluded that the LIP reacts with peroxynitrite and decreases the yield of peroxynitrite-derived oxidants. This was a paradigm-shifting finding in LIP biochemistry and raised many questions. In this follow-up study, we address fundamental properties of the interaction between the LIP and peroxynitrite by using the same cellular model and fluorescence methodology. We have identified that the reaction between the LIP and peroxynitrite has catalytic characteristics, and we have estimated that the rate constant of the reaction is in the range of 10 to 10 Ms. Together, these observations suggest that the LIP represents a constitutive peroxynitrite reductase system in RAW 264.7 cells.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466499 | PMC |
| http://dx.doi.org/10.3390/biom11091331 | DOI Listing |
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