4mC-RF: Improving the prediction of 4mC sites using composition and position relative features and statistical moment.

N4-methylcytosine (4 mC) is an important epigenetic modification that occurs enzymatically by the action of DNA methyltransferases. 4 mC sites exist in prokaryotes and eukaryotes while playing a vital role in regulating gene expression, DNA replication, and cell cycle. The efficient and accurate prediction of 4 mC sites has a significant role in the insight of 4 mC biological properties and functions. Therefore, a sequence-based predictor is proposed, namely 4 mC-RF, for identifying 4 mC sites through the integration of statistical moments along with position, and composition-dependent features. Relative and absolute position-based features are computed to extract optimal features. A popular machine learning classifier Random Forest was used for training the model. Validation results were obtained through rigorous processes of self-consistency, 10-fold cross-validation, Independent set testing, and Jackknife yielding 95.1%, 95.2%, 97.0%, and 94.7% accuracies, respectively. Our proposed model depicts the highest prediction accuracies as compared to existing models. Subsequently, the developed 4 mC-RF model was constructed into a web server. A significant and more accurate predictor of 4 mC Methylcytosine sites helps experimental scientists to gather faster, efficient, and cost-effective results.