Chemical microscopy combines high-resolution emission spectra with Abbe-limited spatial resolution and is used for studies of inhomogeneous samples at the (sub-)micronscale. The spatial distinction of multiple Eu(III) coordination sites allows for a comprehensive understanding of environmental samples and highlights the applicability of Eu(III) as a molecular probe in medicine and biology.
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http://dx.doi.org/10.1039/d1an01449h | DOI Listing |
Pest Manag Sci
January 2025
Dpto. Microbiología, Facultad de Ciencias, Universidad de Málaga, Málaga, Spain.
Background: Chitin is a crucial component of fungal cell walls and an effective elicitor of plant immunity; however, phytopathogenic fungi have developed virulence mechanisms to counteract the activation of this plant defensive response. In this study, the molecular mechanism of chitin-induced suppression through effectors involved in chitin deacetylases (CDAs) and their degradation (EWCAs) was investigated with the idea of developing novel dsRNA-biofungicides to control the cucurbit powdery mildew caused by Podosphaera xanthii.
Results: The molecular mechanisms associated with the silencing effect of the PxCDA and PxEWCAs genes were first studied through dsRNA cotyledon infiltration assays, which revealed a ≈80% reduction in fungal biomass and a 50% decrease in gene expression.
Small Methods
January 2025
Dept. Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, CB3 0AS, UK.
The integration of Machine Learning (ML) with super-resolution microscopy represents a transformative advancement in biomedical research. Recent advances in ML, particularly deep learning (DL), have significantly enhanced image processing tasks, such as denoising and reconstruction. This review explores the growing potential of automation in super-resolution microscopy, focusing on how DL can enable autonomous imaging tasks.
View Article and Find Full Text PDFSensors (Basel)
January 2025
State Key Laboratory of Materials Processing and Die & Mould Technology, School of Materials Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.
This study has investigated the effects of different annealing temperatures on the microstructure, chemical composition, phase structure, and piezoelectric properties of ZnO films. The analysis focuses on how annealing temperature influences the oxygen content and the preferred c-axis (002) orientation of the films. It was found that annealing significantly increases the grain size and optimizes the columnar crystal structure, though excessive high-temperature annealing leads to structural degradation.
View Article and Find Full Text PDFSensors (Basel)
December 2024
Center for Experimental Chemistry Education of Shandong University, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China.
In this study, a simple and easy synthesis strategy to realize the modification of AuHgPt nanoalloy materials on the surface of ITO glass at room temperature is presented. Gold nanoparticles as templates were obtained by electrochemical deposition, mercury was introduced as an intermediate to form an amalgam, and then a galvanic replacement reaction was utilized to successfully prepare gold-mercury-platinum (AuHgPt) nanoalloys. The obtained alloys were characterized by scanning electron microscopy, UV-Vis spectroscopy, X-ray photoelectron spectroscopy and X-ray diffraction techniques.
View Article and Find Full Text PDFInt J Mol Sci
January 2025
School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510275, China.
Objective: The objective of this study was to explore the possibility of treating heart failure in rats by delivering mRNA of 24-dehydrocholesterol reductase (DHCR24) into the body through lipid nanoparticles (LNPs).
Methods: We established a heart failure rat model using doxorubicin. The experiment was divided into blank, model, mRNA stock solution cardiac injection, mRNA stock solution intravenous injection, LNP-mRNA stock solution cardiac injection, and LNP-mRNA stock solution intravenous injection groups.
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