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Antibacterial and cytotoxic activities of undescribed cassiaric acid and other constituents from stem barks. | LitMetric
A new lupane-type triterpene, 2,3-dihydroxylupan-29-oic acid (), and one new ceramide derivative: (2,2',3,4,57',11,12',20)--[2'-hydroxyoctadeca-6,11-dienoyl]-2-aminohexacosa-11,20-diene-1,3,4,5-tetrol () were isolated from the ethyl acetate fraction obtained from the methanol extract of the stem barks of together with seven known compounds. Their structures were characterized using two-dimensional NMR, mass spectrometry, and compared with reported data. To date, this is the first report of the isolation of a multiple double bonds sphingolipid type from this genus. The ethyl acetate extract and selected isolates were examined for antimicrobial and cytotoxic activities . Betulinaldehyde () has shown to be active against all bacterial strains whereas, cassiaric acid ( and betulinic acid ( have demonstrated to be moderately active. In addition, cassiaric acid () showed the best cytotoxic result against HeLa and MCF-7 cell lines tested with IC 75.00 µM, while lupeol ( and betulinic acid ( displayed weak cytotoxicity at 100.00 µM.
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