Voltammetric techniques have been considered as an important analytical tool applied to the determination of trace concentrations of many biological molecules including ascorbic acid. In this paper, ascorbic acid was detected by square wave voltammetry, using graphene oxide paste as a working electrode, modified by a film of a manganese(II) complex compound. Various factors, such as the effect of pH, affecting the response characteristics of the modified electrode were investigated. The relationship between the peak height and ascorbic acid concentration within the modified working electrode was investigated, using the calibration graph. The equation of the calibration graph was found to be: I = 0.0550 + 0.155 with = 0.9998, where I is the SWV current and γac is the mass concentration of ascorbic acid. The LOD and LOQ of the proposed method were determined to be 1.288 μg/L and 3.903 μg/L, respectively. Several compounds, such as riboflavin, biotin, and ions, such as Fe and Cu, were tested and it seemed that they did not interfere with the analytic signal. The proposed procedure was successfully applied in the determination of ascorbic acid in Rosa canina hips.
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| http://dx.doi.org/10.3390/bios11090294 | DOI Listing |
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