AI Article Synopsis

  • - Nanoscale electrophoresis offers a method for separating single molecules, like DNA/RNA nucleobases, which could enable advanced sequencing techniques through label-free detection of nucleotides based on their electrophoretic mobility.
  • - The study introduces a novel dual nanopore sensor that can differentiate four types of 2-deoxyribonucleoside 5'-monophosphates (dNMPs) without labels by measuring transient current as the molecules pass through nanometer-scale pores in a nanochannel.
  • - This sensor demonstrates varying accuracy in distinguishing dNMPs depending on the length of the nanochannel, with the potential for broad application in label-free detection of other molecules and particles by adjusting its design and

Article Abstract

Nanoscale electrophoresis allows for unique separations of single molecules, such as DNA/RNA nucleobases, and thus has the potential to be used as single molecular sensors for exonuclease sequencing. For this to be envisioned, label-free detection of the nucleotides to determine their electrophoretic mobility (i.e., time-of-flight, TOF) for highly accurate identification must be realized. Here, for the first time a novel nanosensor is shown that allows discriminating four 2-deoxyribonucleoside 5'-monophosphates, dNMPs, molecules in a label-free manner by nanoscale electrophoresis. This is made possible by positioning two sub-10 nm in-plane pores at both ends of a nanochannel column used for nanoscale electrophoresis and measuring the longitudinal transient current during translocation of the molecules. The dual nanopore TOF sensor with 0.5, 1, and 5 µm long nanochannel column lengths discriminates different dNMPs with a mean accuracy of 55, 66, and 94%, respectively. This nanosensor format can broadly be applicable to label-free detection and discrimination of other single molecules, vesicles, and particles by changing the dimensions of the nanochannel column and in-plane nanopores and integrating different pre- and postprocessing units to the nanosensor. This is simple to accomplish because the nanosensor is contained within a fluidic network made in plastic via replication.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8542607PMC
http://dx.doi.org/10.1002/smll.202102567DOI Listing

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