AI Article Synopsis
- Organoid culture is a new 3D cell culture technique that has many uses in tissue engineering but poses challenges in histological analysis.
- This study presents an easy and cost-effective method to create both formalin-fixed paraffin embedded (FFPE) and frozen organoid blocks, simplifying the sample preparation process.
- The technique allows for efficient processing of even small quantities of organoids and requires no specialized equipment, making it accessible for routine histological analysis in standard pathology labs.
Article Abstract
Organoid culture is a recently developed in vitro three-dimensional (3D) cell culture technology. It has wide applications in tissue engineering studies. However, histological analysis of organoid is quite complex and tedious for researchers. This study proposes a user-friendly, affordable and efficient method for making formalin-fixed paraffin embedded (FFPE) organoid blocks and Optimal Cutting Temperature compound (OCT) embedded frozen organoid blocks. This method implements a key pre-embedding step for preparing paraffin embedded organoid blocks, which could concentrate organoid together without damaging or loss of samples. This method could be used to process even a small number of organoids with high efficiency. In addition, with minor modifications, the method is readily applied for OCT embedded organoid blocks. The slides generated were ready for H&E staining, immunohistochemistry staining and immunofluorescent staining. The method described in this study can be easily used for routine histological analysis of organoid, and could be performed in general pathology labs and requires no dedicated equipment and reagent.
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| http://dx.doi.org/10.1002/term.3248 | DOI Listing |
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