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Ion exchange chromatography of biotherapeutics: Fundamental principles and advanced approaches.
J Chromatogr A
January 2025
School of Pharmaceutical Sciences, University of Geneva, CMU - Rue Michel Servet 1, 1211 Geneva 4, Switzerland; Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, CMU - Rue Michel Servet 1, 1211 Geneva 4, Switzerland. Electronic address:
Ion exchange chromatography (IEX) is an important analytical technique for the characterization of biotechnology-derived products, such as monoclonal antibodies (mAbs) and more recently, cell and gene therapy products such as messenger ribonucleic acid (mRNA) and adeno-associated viruses (AAVs). This review paper first outlines the basic principles and separation mechanisms of IEX for charge variant separation of biotherapeutics, and examines the different elution modes based on salt or pH gradients. It then highlights several recent trends when applying IEX for the characterization of biotechnology-derived products, including: i) the effective use of pH gradients, ii) the improvement of selectivity by using organic solvents in the mobile phase, multi-step gradients, or by combining ion pairing and ion exchange, and iii) the increase in analytical throughput using ultra-short columns or automated screening of conditions.
View Article and Find Full Text PDFGoals bias face perception.
J Exp Psychol Gen
January 2025
Department of Cognitive and Psychological Sciences, Brown University.
Faces-the most common and complex stimuli in our daily lives-contain multidimensional information used to infer social attributes that guide consequential behaviors, such as deciding who to trust. Decades of research illustrates that perceptual information from faces is processed holistically. An open question, however, is whether goals might impact this perceptual process, influencing the encoding and representation of the complex social information embedded in faces.
View Article and Find Full Text PDFMultidimensional morphological analysis of live sperm based on multiple-target tracking.
Comput Struct Biotechnol J
December 2024
Department of Assisted Reproduction, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, China.
Manual semen evaluation methods are subjective and time-consuming. In this study, a deep learning algorithmic framework was designed to enable non-invasive multidimensional morphological analysis of live sperm in motion, improve current clinical sperm morphology testing methods, and significantly contribute to the advancement of assisted reproductive technologies. We improved the FairMOT tracking algorithm by incorporating the distance and angle of the same sperm head movement in adjacent frames, as well as the head target detection frame IOU value, into the cost function of the Hungarian matching algorithm.
View Article and Find Full Text PDFTuning surface hydrophilicity of a BiVO photoanode through interface engineering for efficient PEC water splitting.
RSC Adv
January 2025
School of Electronic Engineering, Guangxi Key Laboratory of Multidimensional Information Fusion for Intelligent Vehicles, Guangxi University of Science and Technology Liuzhou 545000 China
This study presents a novel approach to enhance photoelectrochemical (PEC) water oxidation by integrating cobalt phthalocyanine (CoPc) with bismuth vanadate (BVO) a direct solvothermal method. The as-prepared BVO@CoPc photoanode demonstrated a photocurrent density of 4.0 mA cm at 1.
View Article and Find Full Text PDFLower respiratory tract microbiome dysbiosis impairs clinical responses to immune checkpoint blockade in advanced non-small-cell lung cancer.
Clin Transl Med
January 2025
Department of Cell Biology, National Translational Science Center for Molecular Medicine, Fourth Military Medical University, Xi'an, China.
Background: Gut microbiome on predicting clinical responses to immune checkpoint inhibitors (ICIs) has been discussed in detail for decades, while microecological features of the lower respiratory tract within advanced non-small-cell lung cancer (NSCLC) are still relatively vague.
Methods: During this study, 26 bronchoalveolar lavage fluids (BALF) from advanced NSCLC participants who received immune checkpoint inhibitor monotherapy were performed 16S rRNA sequencing and untargeted metabolome sequencing to identify differentially abundant microbes and metabolic characteristics. Additionally, inflammatory cytokines and chemokines were also launched in paired BALF and serum samples by immunoassays to uncover their underlying correlations.
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