The European Union banned the usage of antibiotic growth promoters in animal production. The probiotic microorganism of the genus appeared to be an attractive candidate to replace antibiotics. The DSM 29784 is one of these strains. To date, the probiotic effect has not been completely understood, but it is supposed that the effect depends on metabolites of the microorganism. Imaging high-performance thin-layer chromatography (HPTLC) is a powerful tool to visualize differences in the metabolite profile of bacteria with high genetic similarity to allow a better understanding of the probiotic effect. In comparison to other bacteria, especially these bacterial cells were more robust to harsh cultivation conditions and produced a higher level of antioxidants or bioactive substances such as surfactin. HPTLC enabled the comparison of pure cell cultures to the spore cultivation in the feed, and the results explain and support the probiotic effect.
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Unraveling the benefits of DSM 29784 poultry probiotic through its secreted metabolites: an approach.
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European Laboratory of Innovation Science & Expertise (ELISE). Adisseo France S.A.S., Saint Fons, France.
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- The probiotic 29784 (Bs29784) supports chicken intestinal health by producing metabolites like hypoxanthine, niacin, and pantothenate, enhancing resilience and performance through immune response, barrier function, and microbiota modulation.
- Experimental models showed that Bs29784 vegetative cells significantly reduced inflammation and improved intestinal integrity better than spores, while individual metabolites had distinct positive effects on inflammation and cell growth.
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Strain-specific quantification of probiotic Bacillus subtilis in feed by imaging high-performance thin-layer chromatography.
J Chromatogr A
August 2022
Institute of Nutritional Science, Chair of Food Science, and Interdisciplinary Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, Giessen 35392, Germany. Electronic address:
The European Union has banned the use of antibiotic growth promoters in animal production, which has led to increased use of probiotic microorganisms. These feed additives result in higher costs for farmers, which is why the demand for a quality control system to quantify probiotics in feeds has increased in recent years. Imaging high-performance thin-layer chromatography (HPTLC) was proven to be a robust method for determining the probiotic Bacillus subtilis DSM 29784 strain based on the production of selective bacterial metabolites and thus characteristic metabolite pattern.
View Article and Find Full Text PDFBacillus subtilis spores in probiotic feed quantified via bacterial metabolite using planar chromatography.
Anal Chim Acta
August 2022
Institute of Nutritional Science, Chair of Food Science, and Interdisciplinary Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany. Electronic address:
Interest in probiotics in animal production has increased due to the European ban on antibiotic growth promoters in 2006. Bacillus subtilis DSM 29784 (B. s.
View Article and Find Full Text PDFEffects of the Probiotic Activity of DSM 29784 in Cultures and Feeding Stuff.
J Agric Food Chem
September 2021
Institute of Nutritional Science, Chair of Food Science, and Interdisciplinary Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, Giessen 35392, Germany.
The European Union banned the usage of antibiotic growth promoters in animal production. The probiotic microorganism of the genus appeared to be an attractive candidate to replace antibiotics. The DSM 29784 is one of these strains.
View Article and Find Full Text PDFSupplemental DSM 29784 and enzymes, alone or in combination, as alternatives for antibiotics to improve growth performance, digestive enzyme activity, anti-oxidative status, immune response and the intestinal barrier of broiler chickens.
Br J Nutr
March 2021
College of Animal Sciences, Zhejiang University, Hangzhou, People's Republic of China.
The present study investigated the effect of Bacillus subtilis DSM 29784 (Ba) and enzymes (xylanase and β-glucanases; Enz), alone or in combination (BE) as antibiotic replacements, on the growth performance, digestive enzyme activity, immune response and the intestinal barrier of broiler chickens. In total, 1200 1-d-old broilers were randomly assigned to five dietary treatments, each with six replicate pens of forty birds for 63 d as follows: (a) basal diet (control), supplemented with (b) 1 × 109 colony-forming units (cfu)/kg Ba, (c) 300 mg/kg Enz, (d) 1 × 109 cfu/kg Ba and 300 mg/kg Enz and (e) 250 mg/kg enramycin (ER). Ba, Enz and BE, similar to ER, decreased the feed conversion rate, maintained intestinal integrity with a higher villus height:crypt depth ratio and increased the numbers of goblet cells.
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