AI Article Synopsis
- A new method has been created to analyze the specificity of auto-reactive antibodies targeting desmoglein 3 (Dsg3) in pemphigus vulgaris patients using a competitive ELISA.
- This method involves a two-stage process where auto-reactive antibodies are initially removed before measuring the remaining antibodies against the full Dsg3 domain.
- The technique may help in personalizing treatment for patients through plasmapheresis by tailoring the immunosorbent used in therapy.
Article Abstract
A method for the analysis of the epitope specificity of auto-reactive antibodies to desmoglein 3 (Dsg3) using competitive ELISA has been developed. It is based on a two-stage solid-phase ELISA with initial "depletion" of auto-reactive antibodies against the studied epitope and subsequent quantitative assessment of antibodies against full-length extracellular domain Dsg3. The proposed approach for assessing the specificity of the autoimmune response in patients with pemphigus vulgaris can provide in the future the possibility to personalize the therapy using plasmapheresis by preliminary selection of the antigenic composition of the extracorporeal immunosorbent.
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| http://dx.doi.org/10.1007/s10517-021-05254-9 | DOI Listing |
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