AI Article Synopsis
- A study on the cyanobacterium PCC 7942 revealed a mechanism that suppresses its biofilm development, focusing on mutants lacking this inhibition.
- Researchers utilized chlorophyll measurements to quantify the biomass of both sessile (attached) and planktonic (free-floating) cells in biofilm-forming strains.
- This approach helps in estimating total biomass via chlorophyll levels and visualizing the degree of biofilm formation by analyzing chlorophyll distribution between the two cell types.
Article Abstract
A self-suppression mechanism of biofilm development in the cyanobacterium PCC 7942 was recently reported. These studies required quantification of biofilms formed by mutants impaired in the biofilm-inhibitory process. Here we describe in detail the use of chlorophyll measurements as a proxy for biomass accumulation in sessile and planktonic cells of biofilm-forming strains. These measurements allow quantification of the total biomass as estimated by chlorophyll level and representation of the extent of biofilm formation by depicting the relative fraction of chlorophyll in planktonic cells.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8413561 | PMC |
| http://dx.doi.org/10.21769/BioProtoc.2406 | DOI Listing |
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