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Microvesicle Isolation from Rat Brain Extract Treated Human Mesenchymal Stem Cells. | LitMetric

Microvesicle Isolation from Rat Brain Extract Treated Human Mesenchymal Stem Cells.

Bio Protoc

Department of Biomedical Sciences, College of Medical Convergence, Catholic Kwandong University, Gangneung-si, Gangwon-do, Republic of Korea.

Published: July 2017

AI Article Synopsis

Article Abstract

Microvesicle (MVs) are submicron-sized membranous vesicles that are either actively released from cells via secretory compartments or shed from cell surface membranes. MVs are generated by many cell types and serve as vehicles that transfer biological information (, protein, mRNA, and miRNA) to distant cells, thereby affecting their gene expression, proliferation, differentiation, and function. Although their physiological functions are not clearly defined, recent studies have shown their therapeutic potential for tissue repair and regeneration. While MVs can be isolated readily from mesenchymal stem cells (MSCs) and other cell types from various sources, the yield of MVs under conventional culture condition is one of the limiting factors for both the functional study as well as molecular analysis. Here, we provide a protocol to increase the yield of microvesicles by preconditioning MSCs with rat brain extract.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8413510PMC
http://dx.doi.org/10.21769/BioProtoc.2375DOI Listing

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