RNA-guided endonucleases (RGENs) have been used for genome editing in various organisms. Here, we demonstrate a simple method for performing targeted mutagenesis and genotyping in a model moss species, , using RGENs. We also performed targeted mutagenesis in a non-model moss, , using a similar method ( Nomura , 2016 ), indicating that this experimental system could be applied to a wide range of mosses species.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8410312 | PMC |
| http://dx.doi.org/10.21769/BioProtoc.2359 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
and synergistically regulate nodulation and affect arbuscular mycorrhiza in .
Front Plant Sci
December 2024
Department of Genetics and Biochemistry, Clemson University, Clemson, SC, United States.
Two symbiotic processes, nodulation and arbuscular mycorrhiza, are primarily controlled by the plant's need for nitrogen (N) and phosphorus (P), respectively. Autoregulation of nodulation (AON) and autoregulation of mycorrhizal symbiosis (AOM) both negatively regulate their respective processes and share multiple components-plants that make too many nodules usually have higher arbuscular mycorrhiza (AM) fungal root colonization. The protein TML (TOO MUCH LOVE) was shown to function in roots to maintain susceptibly to rhizobial infection under low N conditions and control nodule number through AON in .
View Article and Find Full Text PDFTransposon mutagenesis identifies the sspA-sspB operon as essential for serum resistance and virulence in avian pathogenic Escherichia coli.
Vet Microbiol
December 2024
Key Laboratory of Avian Bioproduct Development, Ministry of Agriculture and Rural Affairs, Yangzhou, Jiangsu 225009, PR China; College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu 225009, PR China; Institutes of Agricultural Science and Technology Development, Yangzhou University, Ministry of Agriculture and Rural Affairs, Yangzhou, Jiangsu 225009, PR China. Electronic address:
Avian pathogenic Escherichia coli (APEC) constitutes a significant threat to poultry health worldwide, causing colibacillosis and inflicting substantial economic losses. The ability to resist serum-mediated killing is a key virulence factor enabling APEC to circumvent the host immune system and establish systemic infection. In this study, we employed mariner-based transposon mutagenesis to generate a mutant library of APEC strain E058 and screened for mutants with reduced serum resistance.
View Article and Find Full Text PDFEfficient gene deletion of Integrin alpha 4 in primary mouse CD4 T cells using CRISPR RNA pair-mediated fragmentation.
Front Immunol
December 2024
Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
The functional specialization of CD4 T lymphocytes into various subtypes, including T1 and T cells, is crucial for effective immune responses. T cells facilitate B cell differentiation within germinal centers, while T1 cells are vital for cell-mediated immunity against intracellular pathogens. Integrin α4, a cell surface adhesion molecule, plays significant roles in cell migration and co-stimulatory signaling.
View Article and Find Full Text PDFThe mutation of ent-kaurenoic acid oxidase, a key enzyme involved in gibberellin biosynthesis, confers a dwarf phenotype to cucumber.
Theor Appl Genet
December 2024
State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Weigang Street No.1, Nanjing, 210095, China.
A dwarf mutant with short branches (csdf) was identified from EMS-induced mutagenesis. Bulked segregant analysis sequencing and map-based cloning revealed CsKAO encoding ent-kaurenoic acid oxidase as the causal gene. Plant architecture is the primary target of artificial selection during domestication and improvement based on the determinate function for fruit yield.
View Article and Find Full Text PDFUnlabelled: The cat eye syndrome chromosome region candidate 2 (CECR2) protein is an epigenetic regulator involved in chromatin remodeling and transcriptional control. The CECR2 bromodomain (CECR2-BRD) plays a pivotal role in directing the activity of CECR2 through its capacity to recognize and bind acetylated lysine residues on histone proteins. This study elucidates the binding specificity and structural mechanisms of CECR2-BRD interactions with both histone and non-histone ligands, employing techniques such as isothermal titration calorimetry (ITC), nuclear magnetic resonance (NMR) spectroscopy, and a high-throughput peptide assay.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!